Construction of the new and efficient culture method of human iPS cell-derived hepatic progenitor-like cells
| Project/Area Number |
16K19370
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
TSURUYA Kota 東海大学, 医学部, 助教 (00725377)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ヒトiPS細胞 / 肝前駆細胞 / フィーダーフリー / 細胞表面分子 / IGF-1R |
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| Outline of Final Research Achievements |
Among the analyses of cell surface receptors, we found that proliferative ability of human iPS cell-derived hepatic progenitor-like cells (HPCs) was significantly induced by addition of both Epidermal Growth Factor(EGF) and Neuregulin1(NRG1). Moreover, we revealed that HPCs maintained high proliferative ability on the human laminin-511 fragments as substitute for culturing on mouse embryonic fibroblasts (MEFs) as feeder cells. Based on these results, we established stably and long-term expansion culture system of human iPS cell-derived HPCs.
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Report
(3 results)
Research Products
(2 results)
