Construction of the new and efficient culture method of human iPS cell-derived hepatic progenitor-like cells

Research Project

Project/Area Number	16K19370
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Gastroenterology
Research Institution	Tokai University
Principal Investigator	TSURUYA Kota 東海大学, 医学部, 助教 (00725377)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	ヒトiPS細胞 / 肝前駆細胞 / フィーダーフリー / 細胞表面分子 / IGF-1R
Outline of Final Research Achievements	Among the analyses of cell surface receptors, we found that proliferative ability of human iPS cell-derived hepatic progenitor-like cells (HPCs) was significantly induced by addition of both Epidermal Growth Factor(EGF) and Neuregulin1(NRG1). Moreover, we revealed that HPCs maintained high proliferative ability on the human laminin-511 fragments as substitute for culturing on mouse embryonic fibroblasts (MEFs) as feeder cells. Based on these results, we established stably and long-term expansion culture system of human iPS cell-derived HPCs.