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Generation of higher-quality iPS cells

Research Project

Project/Area Number 16K19429
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cardiovascular medicine
Research InstitutionKyoto University (2017)
Keio University (2016)

Principal Investigator

Kunitomi Akira  京都大学, iPS細胞研究所, 特定研究員 (30570882)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsiPS細胞 / リンカーヒストンH1 / 再生医学
Outline of Final Research Achievements

Embryonic stem cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes, and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents.
Here we show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with Oct4, Sox2, and Klf4 significantly enhanced the efficiency of iPSC generation. H1foo promoted in vitro differentiation characteristics with low heterogeneity in iPSCs. H1foo enhanced the generation of germline competent chimeric mice from iPSCs in a manner similar to that for ESCs. Furthermore, human H1FOO promoted the efficiency of human iPSC generation. These findings indicate that H1foo contributes to the generation of higher-quality iPSCs.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells2016

    • Author(s)
      Kunitomi A, Yuasa S, Sugiyama F, Saito Y, Seki T, Kusumoto D, Kashimura S, Takei M, Tohyama S, Hashimoto H, Egashira T, Tanimoto Y, Mizuno S, Tanaka S, Okuno H, Yamazawa K, Watanabe H, Oda M, Kaneda R, Matsuzaki Y, Nagai T, Okano H, Yagami K, Tanaka M, Fukuda K.
    • Journal Title

      Stem Cell Reports

      Volume: 6 Issue: 6 Pages: 825-833

    • DOI

      10.1016/j.stemcr.2016.04.015

    • NAID

      120007135348

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 卵母細胞特異的リンカーヒストンH1FOOを用いた高品質ヒトiPS細胞の樹立と心筋分化への応用2018

    • Author(s)
      國富晃
    • Organizer
      第5回 東京未来医療フォーラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 卵母細胞特異的リンカーヒストンH1fooによる高品質iPS細胞の樹立2017

    • Author(s)
      國富晃
    • Organizer
      第16回 日本再生医療学会総会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-09
    • Related Report
      2016 Research-status Report
  • [Presentation] H1foo has a pivotal role in qualifying induced pluripotent stem cells2017

    • Author(s)
      國富晃
    • Organizer
      第5回 IRG meeting
    • Place of Presentation
      品川プリンスホテル(東京都港区)
    • Year and Date
      2017-01-07
    • Related Report
      2016 Research-status Report
  • [Presentation] H1foo has a pivotal role in qualifying induced pluripotent stem cells2017

    • Author(s)
      國富晃
    • Organizer
      第15回 幹細胞シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 卵母細胞特異的リンカーヒストンH1fooによる高品質iPS細胞の樹立と心筋分化への応用2016

    • Author(s)
      國富晃
    • Organizer
      第20回 日本適応医学会学術集会
    • Place of Presentation
      東京コンベンションホール(東京都中央区)
    • Year and Date
      2016-12-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 卵母細胞特異的リンカーヒストンH1fooによる高品質iPS細胞の樹立2016

    • Author(s)
      國富晃
    • Organizer
      第39回 日本高血圧学会総会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-10-02
    • Related Report
      2016 Research-status Report
  • [Presentation] H1foo has a pivotal role in qualifying induced pluripotent stem cells2016

    • Author(s)
      Kunitomi A
    • Organizer
      Cell Symposia
    • Place of Presentation
      Berkeley, CA, USA
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新規クロマチンリモデリング因子H1fooによる高品質iPS細胞の樹立2016

    • Author(s)
      國富晃
    • Organizer
      Molecular Cardiovascular Conference II(2016)
    • Place of Presentation
      東京ドームホテル(東京都文京区)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
  • [Remarks] iPS細胞を高品質かつ高効率に作製することに成功

    • URL

      https://www.amed.go.jp/news/release_20160527.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] iPS細胞を高品質かつ高効率に作製することに成功

    • URL

      https://www.keio.ac.jp/ja/press_release/2016/osa3qr000001q0lk.html

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Patent(Industrial Property Rights)] 高品質なiPS細胞の製造方法2016

    • Inventor(s)
      湯浅慎介、福田恵一、國富晃
    • Industrial Property Rights Holder
      湯浅慎介、福田恵一、國富晃
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-07-11
    • Related Report
      2016 Research-status Report
    • Overseas

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Published: 2016-04-21   Modified: 2019-03-29  

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