SLPI is important for influenza pneumonia via macrophage activation

• Project/Area Number: 16K19439
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Tohoku University
• Principal Investigator: Hirano Taizou 東北大学, 大学病院, 医員 (90733832)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: SLPI / インフルエンザ / Reactive Oxygen Species / 幹細胞 / インフルンザ

Outline of Final Research Achievements

We previously reported two important point for the relationship about influenza pneumonia and lung stem cell. First, bronchiolar progenitor cell is more sensitive for influenza virus than other lung cells, Second,secretory leukocyte protease inhibitor (SLP), which is antiprotenase inhibitor, is important for club cell differentation from bronchiolar progenitor cell. From these things, we hypothesis that SLPI is important for influenza pneumonia.First we evaluated that survival rate and lung inflammation of influenza pneumonia model in SLPI KO and WT mice. Lung inflammation but not survival rate is meekly increased increased in SLPI KO mice than WT. We next evaluated that lung macrophage differentiation by using M/M2 method. In SLPI KO mice, M1/M2 differentiation is more activated than WT mice. Last, We evaluated the pathology of activating M1 macrophage. In SLPI KO mice, the level of inflammation cytokines such as TNF　α　and IFNγ is more unregulated than WT mice.

Research Products

• [Journal Article] Production of reactive persulfide species in chronic obstructive pulmonary disease (2017)
  • Author(s): Numakura T, Sugiura H, Akaike T, Ida T, Fujii S, Koarai A, Yamada M, Onodera K, Hashimoto Y, Tanaka R, Sato K, Shishikura Y, Hirano T, Yanagisawa S, Fujino N, Okazaki T, Tamada T, Hoshikawa Y, Okada Y, Ichinose M
  • Journal Title: Thorax Volume: 72 Issue: 12 Pages: 1074-1083
  • DOI: 10.1136/thoraxjnl-2016-209359
  • Peer Reviewed / Open Access
• [Journal Article] Invasive pulmonary mucormycosis: rare presentation with pulmonary eosinophilia. (2017)
  • Author(s): Hirano T, Yamada M, Sato K, Murakami K, Tamai T, Mitsuhashi Y, Tamada T, Sugiura H, Sato N, Saito R, Tominaga J, Watanabe A, Ichinose M.
  • Journal Title: BMC Pulm Med Volume: 17 Issue: 1 Pages: 76-76
  • DOI: 10.1186/s12890-017-0419-1
  • Peer Reviewed / Open Access
• [Journal Article] 自然崩壊型腫瘍崩壊症候群を合併した肺扁平上皮癌の1例 (2017)
  • Author(s): 板倉 康司，平野 泰三，宮内 栄作，井上 彰，杉浦 久敏，一ノ瀬正和
  • Journal Title: 日本呼吸器学会雑誌 Volume: 5
  • Peer Reviewed
• [Journal Article] Two cases of endobronchial aspergilloma complicated with primary and metastatic lung cancer: A case report and literature review. (2016)
  • Author(s): Hirano T, Yamada M, Igusa R, Tanno A, Numakura T, Sakamoto K, Kikuchi T, Ichinose M
  • Journal Title: respiratory investigation Volume: 54 Pages: 211-215
  • Peer Reviewed
• [Journal Article] Two cases of pseudo-achalasia with lung cancer: a case report and short literature review. (2016)
  • Author(s): Hirano T, Miyauchi E, Inoue A, Igusa R, Chiba S, Sakamoto K, Sugiura H, Kikuchi T, Ichinosea M
  • Journal Title: respiratory investigation Volume: 54 Pages: 494-499
• [Funded Workshop] ATS (2016)
  • Place of Presentation: San Francisco,アメリカ
  • Year and Date: 2016-05-19