Role of the ubiquitin-proteasome system in acid excretion of the renal collecting ducts.
| Project/Area Number |
16K19493
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
Izumi Yuichiro 熊本大学, 大学院生命科学研究部(医), 助教 (20736243)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 代謝性アシドーシス / 慢性腎臓病 / ユビキチン‐プロテアソーム系 / レニン‐アンジオテンシンーアルドステロン系 / ユビキチンープロテアソーム系 / アンモニアトランスポーター / レニン－アンジオテンシン－アルドステロン系 / ユビキチン－プロテアソーム系 / 酸塩基平衡 / 腎生理 |
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| Outline of Final Research Achievements |
Nonvolatile acid is generated from diet and cell metabolism, and then excreted to urine through the intercalated cells in renal collecting ducts by aldosterone. We investigated the role of aldosterone in the regulation of Rhesus Blood Group, C Glycoprotein（Rhcg）protein. In vivo study, we found that aldosterone induced the accumulation of Rhcg. Consistent with that, aldosterone directly induced the expression of Rhcg protein in membrane fraction of the intercalated cell line. We further investigated the role of the ubiquitin-proteasome system (UPS), which is activated by metabolic acidosis, in the regulation of Rhcg protein. We found that low pH induced ubiquitination of proteins in the intercalated cell line. Using immunoprecipitation method, we found Rhcg was ubiquitinated. The results suggest that aldosterone and UPS regulate the expression of Rhcg protein through interaction of the two.
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Report
(3 results)
Research Products
(21 results)
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[Presentation] Fludrocortisone time-dependently up-regulates erythropoietin (Epo) mRNA expression via HIF2α pathway in rat kidney.2017
Author(s)
Nonoguchi H, Sato Y, Kawahara K, Yasuoka Y, Izumi Y, Nakayama Y, Inoue H, Nagai T, Nanami M, Nakanishi T, Mukoyama M.
Organizer
The American Society of Nephrology Kidney Week 2017
Related Report
Int'l Joint Research
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[Presentation] In silico and in vitro characterization of low pH-induced genes in intercalated cells of the collecting ducts.2016
Author(s)
Izumi Y, Eguchi K, Matsuo N, Nakayama Y, Inoue H, Nonoguchi H, Kakizoe Y, Kuwabara T, Mukoyama M.
Organizer
The American Society of Nephrology Kidney Week 2016
Place of Presentation
Chicago, IL, USA
Year and Date
2016-11-19
Related Report
Int'l Joint Research
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[Presentation] Involvement of aldosterone and the ubiquitin-proteasome system in the regulation of expression of an ammonia transporter, rhesus blood group C glycoprotein, in the intercalated cells.2016
Author(s)
Eguchi K, Izumi Y, Nakayama Y, Inoue H, Kakizoe Y, Kuwabara T, Matsuo N, Nakagawa T, Mukoyama M.
Organizer
The American Society of Nephrology Kidney Week 2016
Place of Presentation
Chicago, IL, USA
Year and Date
2016-11-19
Related Report
Int'l Joint Research
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[Presentation] Aldosterone and vasopressin are erythropoietic hormones.2016
Author(s)
Nonoguchi H, Izumi Y, Yasuoka Y, Nakayama Y, Nagai T, Nanami M, Nakanishi T, Mukoyama M, Kawahara K.
Organizer
The American Society of Nephrology Kidney Week 2016
Place of Presentation
Chicago, IL, USA
Year and Date
2016-11-18
Related Report
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