The relationship of paternal overnutrition to renal disfunction in offspring.

• Project/Area Number: 16K19502
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Kurume University
• Principal Investigator: Yamada Nana 久留米大学, 医学部, 助教 (70770022)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 腎障害 / 父親の過栄養 / 腎不全 / 脂質 / 糖代謝 / エピジェネティクス

Outline of Final Research Achievements

We examined renal injury of offspring due to paternal overnutrition. Male Sprague-Dawley rats were fed either a standard (N) or a high-fat and high-fructose (HFF)-diet for 6 weeks before mating, and kept each diet during the gestation and lactation period. After 4 weeks postpartum, all the offspring were fed N diet, and followed by 12 weeks. In HFF-offspring, appetite stimulation was recognized, the weight and albuminuria increased from about 8 weeks of age. HFF-offspring developed hyperlipidemia, hyperglycemia, and hypertension. Adiponectin decrease were observed at 16 weeks old in HFF-offspring. Paternal overnutrition may cause impaired glucose tolerance and renal impairment of the offspring in future. To elucidate the mechanism, we focus on adiponectin and investigate. We will evaluate histological glomerular disorders including local adiponectin expression by immunostaining, and add epigenetics analysis.

Research Products

• [Journal Article] RAGE-aptamer Blocks the Development and Progression of Experimental Diabetic Nephropathy. (2017)
  • Author(s): Matsui, T., Higashimoto, Y., Nishino, Y., Nakamura, N., Fukami, K., Yamagishi, S.
  • Journal Title: Diabetes Volume: 66 Issue: 1 Pages: 1683-1695
  • DOI: 10.1038/s41598-018-21176-5
  • NAID: 40021434304
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 1.Maternal exposure to high-fat and high-fructose diet evokes hypoadiponectinemia and kidney injury in rat offspring. (2016)
  • Author(s): Yamada-Obara, Sho-ichi Yamagishi, Kensei Taguchi, Yusuke Kaida, Miyuki Yokoro, Yosuke Nakayama, Ryotaro Ando, Katsuhiko Asanuma, Takanori Matsui, Seiji Ueda, Seiya Okuda, and Kei Fukami.
  • Journal Title: Clinical and Experimental Nephrology Volume: 20 Issue: 6 Pages: 853-861
  • DOI: 10.1007/s10157-016-1265-9
  • Peer Reviewed / Acknowledgement Compliant
• [Book] 臨床と研究 (2016)
  • Author(s): 山田 奈々、深水 圭
  • Total Pages: 4
  • Publisher: 大道学館