| Project/Area Number |
16K19506
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Kanazawa University |
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Principal Investigator |
Ikeda Tokuhei 金沢大学, 医学系, 協力研究員 (30755773)
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| Research Collaborator |
YAMADA Masahito 金沢大学, 医学系, 教授
ONO Kenjiro 昭和大学, 医学部, 教授
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | Alzheimer病 / アミロイドβ / オリゴマー / アルツハイマー病 / 脳脊髄液 / アンギオテンシン / オリゴマー化 / 神経科学 |
|---|
| Outline of Final Research Achievements |
The purpose of this research was to identify inhibitors of amyloid β (Aβ) oligomerization in human cerebrospinal fluid (CSF). CSF was fractionated using centrifugation through a column and size-exclusion high-performance liquid chromatography. Two CSF fractions had inhibitory effects on Aβ oligomerization, as determined using tandem mass spectrometry. We identified 16 peptides with the desired activity: Angiotensins I, II, and III, and known fragments of these peptides. Using photo-induced cross-linking of unmodified proteins (PICUP), we identified 11 peptides with Aβ oligomerization inhibitory activity, including Angiotensins I-III. Thioflavin T fluorescence was used to identify 9 peptides with Aβ fibrillation inhibitory activity, including Angiotensins I-III. The discovery of these peptides contributes to the investigation of the mechanism underlying Alzheimer's disease pathology and the development of therapeutic strategies for Alzheimer’s disease.
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