Construction of new in vitro blood-brain barrier (BBB) and blood-nerve barrier (BNB) model that incorporates multi-culturing system of BBB or BNB components.
| Project/Area Number |
16K19513
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TAKESHITA Yukio 山口大学, 医学部附属病院, 診療助教(4日/週) (70749829)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Blood-Brain Barrier / Blood-Nerve Barrier / laminin / Neuromyelitis optica / transmigration / in vitro model / 炎症細胞浸潤 / 視神経脊髄炎 / ラミニン / Blood-brain barrier / Blood-nerve barrier / migration / 血液脳関門 / 血液神経関門 |
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| Outline of Final Research Achievements |
We construct new Blood-Brain Barrier (BBB) and Blood-Nerve Barrier (BNB) model incorporating multi-cultured system of BBB or BNB specific laminin isoform and conditionally immortalized human BBB or BNB cell lines. Our models are the first in vitro BBB model to evaluate the crosstalk between BBB specific laminin and other BBB components. Next, we successfully generated the first multi-cultured BBB model that allows the transmigration of leukocytes under shear forces.
Using these moldels, we evaluated how Neuromyelitis optica (NMO)-IgG induced the leukocyte transmigration. NMO-IgG induces IL-6 in astrocytes via AQP4 and IL-6 signaling to ECs increases chemokines and drives leukocyte migration.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Effects of Neuromyelitis optica (NMO)-IgG at the blood-brain barrier in vitro.2017
Author(s)
Y Takeshita ,B Obermeier, AC Cotleur, SF Spampinato, F Shimizu, E Yamamoto, Y Sano, YJ Kryzer, VA Lennon, T Kanda, and R.M. Ransohoff.
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Journal Title
Neurol Neuroimmunol Neuroinflammation
Volume: 4
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Effects of neuromyelitis optica-IgG at the blood-brain barrier in vitro.2016
Author(s)
Takeshita Y, Obermeier B, Cotleur AC, Spampinato SF, Shimizu F, Yamamoto E, Sano Y, Kryzer TJ, Lennon VA, Kanda T, Ransohoff RM.
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Journal Title
Neurol Neuroimmunol Neuroinflamm.
Volume: 4
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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