Involvement of gut microbiota changes induced by a westernized diet in the pathogenesis of metabolic syndrome

• Project/Area Number: 16K19538
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Hiroshima University
• Principal Investigator: Okubo Hirofumi 広島大学, 病院(医), 医科診療医 (80770734)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 腸内細菌 / メタボリックシンドローム / インスリン抵抗性

Outline of Final Research Achievements

Matsuda index (MI) was lower in Japanese-American (J-A) than in Native-Japanese (NJ) men with normal glucose tolerance. The gut microbiota composition in J-A men is different, i.e., lower abundance of genera within the phylum Bacteroidetes, from that in NJ men. Furthermore, the ratio between this gut microbiota and MI was positively correlated between the two groups. These results suggested that a westernized diet induces gut microbiota changes, which might be involved in insulin resistance.
High-fat high-sucrose diet (HFHSD)-fed mice showed gut microbiota changes and colon inflammation. Miglitol treatment partially corrected these changes in the gut environment and showed a protective effect against the development of HFHSD-induced non-alcoholic steatohepatitis. Thus, gut environment might be a therapeutic target for metabolic syndrome.

Research Products

• [Journal Article] Gut microbiota as a therapeutic target for metabolic disorders. (2018)
  • Author(s): Okubo H, Nakatsu Y, Kushiyama A, Yamamotoya T, Matsunaga Y, Inoue MK, Fujishiro M, Sakoda H, Ohno H, Yoneda M, Ono H, Asano T.
  • Journal Title: Curr Med Chem. Volume: 25 Issue: 9 Pages: 984-1001
  • DOI: 10.2174/0929867324666171009121702
  • Peer Reviewed / Open Access
• [Journal Article] Effect of miglitol on the suppression of nonalcoholic steatohepatitis development and improvement of the gut environment in a rodent model (2017)
  • Author(s): Kishida Yumi, Okubo Hirofumi, Ohno Haruya, Oki Kenji, Yoneda Masayasu
  • Journal Title: Journal of Gastroenterology Volume: Epub ahead of print Issue: 11 Pages: 1180-1191
  • DOI: 10.1007/s00535-017-1331-4
  • Peer Reviewed / Open Access
• [Presentation] 日本人と日系米人における腸内細菌叢の違いと肥満の関連 (2017)
  • Author(s): 山本 祐太郎, 大久保 博史, 大野 晴也, 沖 健司, 米田 真康
  • Organizer: 日本糖尿病学会中国四国地方会第55回総会
• [Presentation] Gut microbiota as a therapeutic target for non-alcoholic steatohepatitis (2017)
  • Author(s): Hirofumi Okubo, Yumi Kishida, Haruya Ohno, Kenji Oki, Masayasu Yoneda
  • Organizer: World conference on prebiotics and probiotics 2017
  • Int'l Joint Research
• [Presentation] Miglitol投与による腸内細菌を含めた腸内環境の変化を介した非アルコール性脂肪肝炎発症抑制機構の検討 (2016)
  • Author(s): 岸田祐美、大久保博史、前田修作、大野晴也、沖健司、米田真康
  • Organizer: 第59回日本糖尿病学会年次学術集会
  • Place of Presentation: 京都
  • Year and Date: 2016-05-19