Functional analyses of tisuue engneered thyroid gland fabricated with human iPS cells derived-thyrocytes
Project/Area Number |
16K19561
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Endocrinology
|
Research Institution | Tokyo Women's Medical University |
Principal Investigator |
Arauchi Ayumi 東京女子医科大学, 医学部, 研究生 (40724141)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 甲状腺 / ヒトiPS細胞 / 再生医療 / 分化誘導 / 移植・再生医療 |
Outline of Final Research Achievements |
The replacement of regenerated thyroid follicular cells (TFCs) is a promising therapeutic strategy for patients with hypothyroidism. Differentiation of human induced pluripotent stem cells (iPSCs) to TFCs was induced using a bioreactor developed with clinical application and we analyzed these cells genetically and histologically. At first, suspension culture with Activin A treatment produced definitive endodermal cells that expressed Sox17 and FoxA2 in the RT-PCR and flow cytometric analysis. Further treatment with thyroid-stimulating hormone (TSH) induced TFCs expressing thyroid transcription factors Pax8, Nkx2-1 and various types of thyroid proteins including TSH receptor, sodium-iodide symporter, thyroglobulin and thyroid peroxidase. Interestingly, differentiated cells secreted free thyroxine in vitro. These results indicate successful differentiation of human iPSCs to functional TFCs that may enable us to fabricate thyroid tissues for regenerative medicine and disease models.
|
Report
(3 results)
Research Products
(3 results)