| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Monosomy 7 and interstitial deletion of 7q is a well-recognized nonrandom chromosomal abnormality frequently found among patients with myelodysplastic syndromes and myeloid leukemias. To understand the effects of lacking large deletion on chromosome 7 in hematopoietic cell differentiation, I constructed in vitro model systems using human induced pluripotent stem (iPS) cells and the genome editing system. I suceeded in generating iPS cells carrying 7q that lacks various large regions using the CRISPR/Cas9 genome editing system, and plan to induce these cells to differentiate into multiple hematopoietic cells via the method of the formation of embryoid bodies to examine the hematopoietic function.
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