Epigenetics analysis of HTLV-1 provirus for pathogenesis of ATL
| Project/Area Number |
16K19580
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kumamoto University |
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Principal Investigator |
Katsuya Hiroo 熊本大学, エイズ学研究センター, 特任助教 (80632041)
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| Co-Investigator(Renkei-kenkyūsha) |
SATOU Yorifumi 熊本大学, エイズ学研究センター, 教授 (70402807)
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| Research Collaborator |
MIYAZATO Paola 熊本大学, 国際先端医学研究機構, 特任助教 (90573105)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | HTLV-1 / 成人T細胞白血病-リンパ腫 / エンハンサー / ChIP-seq / 転写因子 / 内科 / ウイルス |
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| Outline of Final Research Achievements |
The nucleosome-free region (NFR) was found near the 3’LTR of HTV-1 provirus by MNase-sequence in both an ATL cell line and PBMCs from the ATL patients. Chromatin immunoprecipitation sequencing (ChIP-seq) showed the histone modification pattern of enhancer regions in the NFR, such as H3K4me1, H3K4me2, and H3K27Ac. The enhancer function of the NFR was also confirmed by Luciferase Reporter Assay. Furthermore, the binding of transcriptional factors, SRF and ELK-1, was detectable in the NFR by ChIP-seq and electrophoretic mobility shift assay. These data suggests that the NFR in HTLV-1 provirus plays a role of enhancer for HBZ expression, which is reverse-transcribed from 3’LTR, and might contribute to persistent infection in infected individuals.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Prognostic index for chronic- and smoldering-type adult T-cell leukemia-lymphoma2017
Author(s)
Katsuya Hiroo、Shimokawa Mototsugu、Ishitsuka Kenji、Kawai Kazuhiro、Amano Masahiro、Utsunomiya Atae、Hino Ryosuke、Hanada Shuichi、Jo Tatsuro、Tsukasaki Kunihiro、Moriuchi Yukiyoshi、Sueoka Eisaburo、Yoshida Shinichiro、Suzushima Hitoshi、Miyahara Masaharu、Yamashita Kiyoshi、Eto Tetsuya、Suzumiya Junji、Tamura Kazuo
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Journal Title
Blood
Volume: 130
Issue: 1
Pages: 39-47
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A prognostic index for chronic- and smoldering-type adult T-cell leukemia-lymphoma2017
Author(s)
Katsuya H, Shimokawa M, Ishitsuka K, Kawai K, Amano M, Utsunomiya A, Hino R, Hanada S, Jo T, Tsukasaki K, Moriuchi Y, Sueoka E, Yoshida S, Suzushima H, Miyahara M, Yamashita K, Eto T, Suzumiya J, Tamura K.
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Journal Title
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Possible involvement of YB-1 in abnormal nuclear shape of adult T-cell leukemia-Iymphoma cells2017
Author(s)
Misaki Kakoki, Hiroo Katsuya, Takafumi Noguchi, Yuki Inada, Paola Miyazato, Misaki Matsuo, Benjy Tan Jek Yang, Islam Mohammad Saiful, Hiroyuki Hata, Tokio Tani, Yorifumi Satou
Organizer
第4回日本HTLV-1学会学術集会
Related Report
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[Presentation] Possible involvement of YB-1 in abnormal nuclear shape of adult T-cell leukemia-Iymphoma cells2017
Author(s)
Misaki Kakoki, Hiroo Katsuya, Takafumi Noguchi, Yuki Inada, Paola Miyazato, Misaki Matsuo, Masahito Tokunaga, Atae Utsunomiya, Hiroyuki Hata, Tokio Tani, Yorifumi Satou
Organizer
第７９回日本血液学会学術集会
Related Report
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[Presentation] Highly sensitive viral RNA-seq is a powerful tool to analyze the viral transcripts in HTLV-1-infected individuals2017
Author(s)
Yuki Inada, Hiroo Katsuya, Paola Miyazato, Benjy Tan Jek Yang, Saori Iwase, Misaki Kakoki, Misaki Matsuo, Islam Mohammad Saiful, Masahito Tokunaga, Hiroyuki Hata, Yoshihisa Yamano, Atae Utsunomiya, Yorifumi Satou
Organizer
The 59th American Society of Hematology
Related Report
Int'l Joint Research
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