Epstein-Barr virus infection-induced inflammasome activation in human leukocytes.
Project/Area Number |
16K19638
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pediatrics
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Research Institution | Nagoya University |
Principal Investigator |
TORII Yuka 名古屋大学, 医学系研究科, 特別研究員(RPD) (00770281)
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Research Collaborator |
KAWADA Junichi
MURATA Takayuki
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | Epstein-Barr virus / Inflammasomes / interleukin-1β / EBV / インフラマソーム / 単球 / ウイルス / 自然免疫 |
Outline of Final Research Achievements |
Inflammasomes are cytoplasmic sensors that regulate the caspase-1 activation and the secretion of interleukin-1β (IL-1β) or interleukin-18 (IL-18) in response to foreign molecules. Inflammasome activation in human leukocytes during Epstein-Barr virus (EBV) infection was investigated. It was shown that EBV could infect not only to B lymphocyte cell lines, but also to T lymphocyte and monocyte cell lines. EBV infection induced caspase-dependent IL-1β production in monocyte cell lines and primary human monocytes, while not in B-cell or T-cell lines. To identify the sensor molecule, we examined the mRNA and the protein levels of NLR family pyrin domain-containing 3 (NLRP3), absent in melanoma 2 (AIM2), and interferon-inducible protein 16 (IFI16). Increased AIM2 levels were observed in EBV-infected monocyte cell lines. Our results suggest that EBV infection of human monocytes induces caspase-1-dependent IL-1β production, and that AIM2 is involved in this response.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Comprehensive detection of pathogens in immunocompromised children with bloodstream infections by next-generation sequencing2018
Author(s)
Horiba, K. Kawada, J. Okuno, Y. Tetsuka, N. Suzuki, T. Ando, S. Kamiya, Y. Torii, Y. Yagi, T. Takahashi, Y. Ito, Y.
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Journal Title
Sci Rep
Volume: 8
Issue: 1
Pages: 3784-3784
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Metabolome analysis reveals the association between the kynurenine pathway and human herpesvirus 6 encephalopathy in immunocompetent children2017
Author(s)
Torii, Y. Kawano, Y. Sato, H. Fujimori, T. Sasaki, K. Kawada, J. Takikawa, O. Lim, C. K. Guillemin, G. J. Ohashi, Y. Ito, Y.
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Journal Title
Metabolomics
Volume: 13
Issue: 11
Pages: 126-126
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comprehensive detection of viruses in pediatric patients with acute liver failure using next-generation sequencing2017
Author(s)
Suzuki, T. Kawada, J. Okuno, Y. Hayano, S. Horiba, K. Torii, Y. Takahashi, Y. Umetsu, S. Sogo, T. Inui, A. Ito, Y.
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Journal Title
J Clin Virol
Volume: 69
Pages: 67-72
DOI
Related Report
Peer Reviewed
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[Journal Article] Primary psoas abscess caused by group A streptococcus in a child: Case report with microbiologic findings2016
Author(s)
Kamiya Y, Hasegawa T, Takegami Y, Horiba K, Ando S, Torii Y, Kidokoro H, Kato T, Natsume J, Kawada JI, Ito Y
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Journal Title
J Infect Chemother
Volume: 22
Issue: 12
Pages: 811-814
DOI
Related Report
Peer Reviewed
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[Journal Article] Tofacitinib induces G1 cell-cycle arrest and inhibits tumor growth in Epstein-Barr virus-associated T and natural killer cell lymphoma cells2016
Author(s)
Ando S, Kawada J, Watanabe T, Suzuki M, Sato Y, Torii Y, Asai M, Goshima F, Murata T, Shimizu N, Ito Y, Kimura H
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Journal Title
Oncotarget
Volume: 7
Issue: 47
Pages: 76793-76805
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Risk Factors for Viral Infections in Pediatric Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation.2017
Author(s)
Atsushi Narita, Yoshinori Ito, Yuka Torii, Juinichi Kawada,, Kyogo Suzuki, Eri Nishikawa, Nozomu Kawashima, Yusuke Okuno, Hideki Muramatsu, Nobuhiro Nishio, Asahito Hama, Seiji Kojima, Yoshiyuki Takahashi et al
Organizer
59th ASH Annual Meeting and Exposition
Related Report
Int'l Joint Research
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