Role of DENND2A for development of neuroblastoma

• Project/Area Number: 16K19644
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Kobe University
• Principal Investigator: YAMAMOTO NOBUYUKI 神戸大学, 医学研究科, 医学研究員 (20596043)
• Research Collaborator: Nishimura Noriyuki 神戸大学, 大学院, 特命教授 (00322719) ; Mori Takeshi 神戸大学, 大学院, 特命講師 ; Kosaka Yoshiyuki 兵庫県立こども病院, 血液腫瘍内科部長
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経芽腫 / がん幹細胞 / DENND2A / Rab9B / 細胞内小胞輸送 / DENNドメイン蛋白質 / Rabファミリー低分子量G蛋白質

Outline of Final Research Achievements

Neuroblastoma is a typical recurrent and refractory tumor in children and its long-term survival remains less than 40%. Neuroblastoma relapse is caused by activation of chemo-resistant cancer stem cells (CSCs). Key regulators of intracellular vesicle trafficking, DENN domain proteins, were implicated in this process. In this study, a member of DENND domain proteins DENND2A and its target Rab family small G protein Rab9B were found to be involved in the development and progression of neuroblastoma.

Research Products

• [Journal Article] Early detection of tumor relapse/re-growth by consecutive minimal residual disease monitoring in high-risk neuroblastoma patients. (2016)
  • Author(s): Hirase S, Saitoh A, Hartomo TB, Kozaki A, Yanai T, Hasegawa D, Kawasaki K, Kosaka Y, Matsuo M, Yamamoto N, Mori T, Hayakawa A, Iijima K, Nishio H, and Nishimura N.
  • Journal Title: Oncol Let Volume: 12 Issue: 2 Pages: 1119-1123
  • DOI: 10.3892/ol.2016.4682
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Involvement of DENND2A and Rab9B in the progression of neuroblastoma (2017)
  • Author(s): NOBUYUKI YAMAMOTO
  • Organizer: 3rd Asia-Pacific International Symposium of Neuroblastoma
  • Int'l Joint Research
• [Presentation] DENN domain protein DENND2A regulates the progression of neuroblastoma (2016)
  • Author(s): Nobuyuki Yamamoto, Khin Kyaemon Thwin, Jun-ya Fujimura, Keita Nakanishi, Takeshi Mori, Akira Hayakawa, Hisahide Nishio, Masafumi Matsuo, Daiichiro Hasegawa, Keiichiro Kawasaki, Yoshiyuki Kosaka, Kazumoto Iijima, and Noriyuki Nishimura
  • Organizer: Advance in Neuroblastoma Research Conference 2016
  • Place of Presentation: Cairns Convention Centre, Cairns, Australia
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] Rab6B mediates the progression of neuroblastoma through the interaction with MTMR5. (2016)
  • Author(s): Nishimura N, Pham TVH, Thwin KK, Yamamoto N, Mori T, Hayakawa A, Nishio H, Matsuo M, Hasegawa D, Kawasaki K, Kosaka Y, and Iijima K.
  • Organizer: Advances in Neuroblastoma Research Conference 2016
  • Place of Presentation: Cairns Convention Centre, Cairns, Australia
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] 神経芽腫患者の微小残存病変モニタリング (2016)
  • Author(s): 西村範行, Khin Kyaemon Thwin, 山本暢之, 森健, 早川晶, 長谷川大一郎, 川崎圭一郎, 小阪嘉之, 西尾久英, 飯島一誠.
  • Organizer: 第119回日本小児科学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2016-05-13