Analysis of three-dimensional structure of human sweat glands

• Project/Area Number: 16K19721
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: Kurata Ryuichiro 大阪大学, 薬学研究科, 招へい教員 (50773115)
• Co-Investigator(Renkei-kenkyūsha): SEKIGUCHI Kiyotoshi 大阪大学, 蛋白質研究所・寄附研究部門, 教授 (50187845) ; MUROTA Hiroyuki 大阪大学, 医学系研究科, 准教授 (90363499)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 汗腺 / ３次元構造解析 / ホールマウント免疫染色法 / 発汗 / 収縮機構 / 3次元構造 / 分泌腺 / 筋上皮細胞 / 血管 / 神経 / 3次元構造解析

Outline of Final Research Achievements

A whole-mount staining method was employed to visualize three-dimensional structure of human sweat gland that is unbranched coiled tubule consisting mainly of secretory portion and duct. We found that i) ductal tubule was spatially embraced in secretory tubule; ii) ductal tubule was occasionally bent, while secretory tubule was frequently bent and formed a self-entangled coiled structure; iii) secretory portion was surrounded by myoepithelial cells longitudinally elongated along entangled secretory tubules; iv) nerve fibers wrapped around secretory tubules, but not ductal tubules. These results suggest that multiple myoepithelial cells of sweat gland synchronously contract their secretory portion following innervations.

Research Products

• [Journal Article] Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining. (2017)
  • Author(s): Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, Katayama I, Okada F, Sekiguchi K.
  • Journal Title: PLoS One Volume: 21 Issue: 6 Pages: e0178709-e0178709
  • DOI: 10.1371/journal.pone.0178709
  • NAID: 120006626409
  • Peer Reviewed / Open Access
• [Journal Article] Sweat gland stem cells in gland homeostasis and regeneration (2016)
  • Author(s): Kurata, R., Sekiguchi, S.
  • Journal Title: Household and Personal Care Today Volume: 11 Pages: 39-43
  • Peer Reviewed / Open Access
• [Presentation] Three-dimensional cell shapes and arrangements in human sweat glands at single cell resolution (2017)
  • Author(s): Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, Katayama I, Okada F, Sekiguchi K.
  • Organizer: 2017 Gordon Research Seminar
  • Int'l Joint Research
• [Presentation] Three-dimensional cell shapes and arrangements in human sweat glands at single cell resolution (2017)
  • Author(s): Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, Katayama I, Okada F, Sekiguchi K.
  • Organizer: 2017 Gordon Research Conference
  • Int'l Joint Research
• [Presentation] 細胞外マトリックスと幹細胞ニッチ：生化学者の汗腺研究事始め (2017)
  • Author(s): 関口清俊(連携研究者)
  • Organizer: 第4回汗と皮膚疾患の研究会
• [Presentation] Three-dimensional structural anatomy and sweat gland stem cells in human sweat glands (2016)
  • Author(s): Kurata, R., Fujita, F., Futaki, S., Okada, F., Nakano, I., Tanemura, A., Murota, H., Katayama, I., Sekiguchi, K.
  • Organizer: 第27回CDBミーティング
  • Place of Presentation: 神戸
  • Year and Date: 2016-11-14
• [Presentation] Visualizing the contraction of human eccrine sweat glands ex vivo (2016)
  • Author(s): Nakashima, K., Kurata, R., Fujita, F., Okada, F., Tanemura, A., Murota, H., Katayama, I., Sekiguchi, K.
  • Organizer: 第27回CDBミーティング
  • Place of Presentation: 神戸
  • Year and Date: 2016-11-14
• [Presentation] DEVELOPMENT OF A HUMAN THREE-DIMENSIONAL SWEAT GLAND MODEL TO ASSESS SWEATING (2016)
  • Author(s): Kurata, R., Fujita, F., Futaki, S., Nakano, I., Tanemura, A., Murota, H., Katayama, I., Sekiguchi, K.
  • Organizer: IFSCC 2016 Congress
  • Place of Presentation: ORLANDO FLORIDA
  • Year and Date: 2016-10-29
  • Int'l Joint Research
• [Presentation] ヒト汗腺器官のコイル収縮部とその近傍の血管・神経の三次元的構造解析 (2016)
  • Author(s): 倉田隆一郎、二木杉子、中野伊津子、種村篤、室田浩之、片山一朗、関口清俊
  • Organizer: 第24回日本発汗学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2016-08-27
• [Presentation] 蛍光ライブセルイメージングを用いたヒト汗腺の動態観察 (2016)
  • Author(s): 中島輝恵、倉田隆一郎、藤田郁尚、岡田文裕、種村篤、室田浩之、片山一朗、関口清俊
  • Organizer: 第24回日本発汗学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2016-08-27
• [Presentation] 基調講演：汗腺における筋上皮細胞の研究：汗腺幹細胞の同定から三次元イメージングまで (2016)
  • Author(s): 関口 清俊
  • Organizer: 第24回日本発汗学会総会
  • Place of Presentation: 大阪
• [Remarks] 体温調節を担う汗腺の三次元構造の可視化に成功
  • URL: http://resou.osaka-u.ac.jp/ja/research/2017/20170622_3
• [Remarks] 体温調節を担う汗腺の三次元構造の可視化に成功
  • URL: https://www.mandom.co.jp/release/2017/src/2017062101.pdf
• [Remarks] 先端化粧品科学共同研究講座 倉田隆一郎招へい教員らの論文がPLOS ONE 誌に掲載されました
  • URL: http://www.phs.osaka-u.ac.jp/information/topics_detail.cgi?id=1498183064
• [Remarks] 体温調節を担う汗腺の三次元構造の可視化に成功
  • URL: http://www.protein.osaka-u.ac.jp/category/achievement/joint-research
• [Remarks] 株式会社マンダム [ニュースリリース] ヒトの汗腺幹細胞を発見し、生体外での汗腺様構造体の再生に成功
  • URL: http://www.mandom.co.jp/
• [Remarks] 大阪大学蛋白質研究所 第24回日本発汗学会総会 基調講演
  • URL: http://www.protein.osaka-u.ac.jp/chemistry/
• [Patent(Industrial Property Rights)] 制汗剤 (2018)
  • Inventor(s): 中島輝恵、倉田隆一郎、藤田郁尚、岡田文裕
  • Industrial Property Rights Holder: 中島輝恵、倉田隆一郎、藤田郁尚、岡田文裕
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-062833
  • Filing Date: 2018
• [Patent(Industrial Property Rights)] 不死化汗腺筋上皮細胞およびその製造方法 (2017)
  • Inventor(s): 中島輝恵、倉田隆一郎、藤田郁尚、岡田文裕、関口清俊
  • Industrial Property Rights Holder: 中島輝恵、倉田隆一郎、藤田郁尚、岡田文裕、関口清俊
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-154503
  • Filing Date: 2017
• [Patent(Industrial Property Rights)] 汗腺の動態の観察方法 (2016)
  • Inventor(s): 中島輝恵、倉田隆一郎、藤田郁尚、関口清俊、他3名
  • Industrial Property Rights Holder: 中島輝恵、倉田隆一郎、藤田郁尚、関口清俊、他3名
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-141740
  • Filing Date: 2016
  • Acquisition Date: 2017
• [Patent(Industrial Property Rights)] 汗腺の動態の観察方法 (2016)
  • Inventor(s): 中島、倉田、藤田、関口、種村、室田、片山
  • Industrial Property Rights Holder: 中島、倉田、藤田、関口、種村、室田、片山
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-141740
  • Filing Date: 2016-07-19