| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In this study, we investigated the effects of NMU on the activation of mouse bone marrow-derived mast cells (BMMC), connective-tissue type mouse mast cells differentiated from BMMC (CTMC), and human skin derived mast cells (HSMC) in vitro. NMU induced the degranulation of CTMC and HSMC, but not BMMC, in a concentration-dependent manner. Although no or only slight NMU receptors (NMUR1) were expressed in CTMC and HSMC, MAS-related G protein coupled receptor-X2 (MRGPRX2) and Mrgprb2, mouse analog of MRGPRX2 were highly expressed in HSMC and in CTMC, respectively, but not in BMMC at mRNA level.
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