A new role of TCR V delta 1+ gamma delta T cells in the pathogenesis of alopecia areata

• Project/Area Number: 16K19731
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kagoshima University
• Principal Investigator: UCHIDA Youhei 鹿児島大学, 医歯学域附属病院, 講師 (30571856)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: γδT細胞 / 円形脱毛症 / TCRVδ1+γδT細胞

Outline of Final Research Achievements

Alopecia areata (AA) is an autoimmune disease. Cytotoxic T-cells mediate premature AA HF induction. In this study, γδ T cells were detected in and around AA HFs. Terminally differentiated effector memory (CD45RA+CD27-) Vδ1 T cells significantly increases in AA patient as compared with healthy donors. CD107a is a marker of cell-activation and degranulation of cytotoxic molecule. There is a marked increase of CD107a+Vδ1 T cells in AA patient. CXCR3 and CXCR4, chemokine receptors for T-cell chemoattractant, were upregulated in AA patients. Furthermore, Vδ1 T cells has reduced expression of PD-1, a negative regulator, in AA patient. These findings suggest the cytotoxic and skin migratory ability escaping from self-tolerance of AA recruiting γδ T-cells.

Research Products

• [Int'l Joint Research] Manchester University/Centre for Dermatology Research(英国)
• [Int'l Joint Research] Münster University/Head of the Laboratory for Hair Research/and Regenerative Medicine(ドイツ)
• [Journal Article] Two cases of severe fever with thrombocytopenia syndrome virus infection. (2018)
  • Author(s): Youhei Uchida、Takuro Kanekura
  • Journal Title: The Jounral of Dermatology Volume: - Issue: 5
  • DOI: 10.1111/1346-8138.14138
  • Peer Reviewed / Open Access
• [Presentation] 脱毛症の免疫学的病態 (2017)
  • Author(s): 内田洋平、Marta Bertolini、 Ralf Paus、金蔵拓郎
  • Organizer: 第47回日本皮膚アレルギー・接触皮膚炎学会総会学術大会・第41回皮膚脈管・膠原病研究会
• [Presentation] Resident dermal Vdelta1+T-cells operate as autoimmunity-promoting stress-sentinels in human skin: Relevance for alopecia areata (2017)
  • Author(s): Youhei Uchida、Jennifer Gherardini, Majid Alam, Aviad Keren, Haiping Zhang, Jérémy Chéret, Akiko Arakawa, Thilo Oelert, Alfredo Rossi, Amos Gilhar, Takuro Kanekura, Marta Bertolini,and Ralf Paus
  • Organizer: 10th, World congress for hair research
  • Int'l Joint Research
• [Presentation] 頭部の乾癬病変に生じた多毛症の一例 (2017)
  • Author(s): 内田洋平、金蔵拓郎
  • Organizer: 第32回日本乾癬学会学術大会
• [Remarks] 鹿児島大学病院皮膚科
  • URL: https://com4.kufm.kagoshima-u.ac.jp/images/contents/jouhoukoukai16020128-208.pdf
• [Funded Workshop] 10th, World congress for hair research (2017)