The role of Toll-like receptor 3 in the pathogenesis of immediate type hypersensitivity

Research Project

Project/Area Number	16K19734
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Dermatology
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Nakamura Naomi 京都府立医科大学, 医学部附属病院, 研究員 (10733058)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	Toll様受容体 / 即時型アレルギー / Toll様受容体３ / Toll様受容体3
Outline of Final Research Achievements	We investigated the role of TLR3 in immediate hypersensitivity in a model of IgE-dependent food anaphylaxis. Wild-type (WT), Tlr3 knockout (KO) mice were epicutaneously sensitized with ovalbumin (OVA) and then challenged orally with OVA. However, there was no significant difference in the serum levels of OVA-specific IgE antibody between OVA-sensitized WT and Tlr3 KO mice. To investigate whether Tlr3 plays a role in the skin barrier dysruption, we mesured IL-33 and TSLP in the tape-stripped mice skin. However, IL-33 and TSLP were not sigmificaly increased. In additoin, the reaction of food anaphylaxis elicited by oral OVA challenge of epicutaneous sensitized WT mice was comparative of that in Tlr3 KO mice.