|Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|Outline of Final Research Achievements
CD326 is a membrane glycoprotein that promotes intercellular adhesion through homophilic interaction. CD326 is expressed at high levels by epidermal Langerhans Cells (LC). Here, we demonstrated that langerin+ dermal dendritic cells (langerin+ dDC) weekly express CD326 on surface. It is also found that langerin+ dDC showed enhanced expression of CD326 in lymph nodes. To study function of CD326 in langerin+ dDC, we developed KO mice in which CD326 is selectively deleted in LC and langerin+ dDC. We explored immunological consequences of lack of CD326 expression on langerin+ dDC by percutaneous immunization assays. We also found that CD326 was expressed at the second layer of stratum granulosum (SG2) layer to seal the intercellular junctions. Activated LC dendrites docking point lost CD326 expression in mice with CD326-deficient LC at SG2. Topically applied biotin penetrated into basal layer in mice with CD326-deficient LCs, suggesting the barrier compromise at LC docking points at SG2.