| Project/Area Number |
16K19745
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Miyagi Prefectural Hospital Organization Miyagi Cancer Center |
|---|
Principal Investigator |
INOUE Yui 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 共同研究員 (30750442)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | プロテインホスファターゼ / Kras / 皮膚腫瘍学 |
|---|
| Outline of Final Research Achievements |
To assess effects of PP6 loss on activated K-ras-induced tumorigenesis in keratinocytes, we established K14-CreERtam/LSL-K-rasG12D/Ppp6cflox/flox mice (exhibiting epidermal-specific tamoxifen-inducible K-rasG12D expression plus Ppp6c deficiency) and K14-CreERtam/LSL-K-rasG12D mice (which showed only epidermal-specific tamoxifen-inducible K-rasG12D expression). Ten independent 4HT-treated K14-CreERtam/LSL-K-rasG12D/Ppp6cflox/flox mice developed lip tumors. Microscopic analysis of lip tumor tissue from the mouse indicated hyperkeratotic papillomatous proliferative lesions. It is noteworthy that a region of squamous cell carcinoma in situ (CIS) sacrificed between days 16 to 20. To examine lip epidermis growth, we measured tissue thickness at 4, 8 and 12 days after 4HT injection. The ratio of lip thickness of K14-CreERtam/LSL-K-rasG12D/Ppp6cflox/flox to that of K14-CreERtam/LSL-K-rasG12D mice was 2.3, 24 and 42, at days 4, 8 and 12, respectively.
|
