Ppp6c deletion promotes mutated RAS-initiated tumor formation in keratinocytes.
Project/Area Number |
16K19745
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
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Research Institution | Miyagi Prefectural Hospital Organization Miyagi Cancer Center |
Principal Investigator |
INOUE Yui 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 共同研究員 (30750442)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | プロテインホスファターゼ / Kras / 皮膚腫瘍学 |
Outline of Final Research Achievements |
To assess effects of PP6 loss on activated K-ras-induced tumorigenesis in keratinocytes, we established K14-CreERtam/LSL-K-rasG12D/Ppp6cflox/flox mice (exhibiting epidermal-specific tamoxifen-inducible K-rasG12D expression plus Ppp6c deficiency) and K14-CreERtam/LSL-K-rasG12D mice (which showed only epidermal-specific tamoxifen-inducible K-rasG12D expression). Ten independent 4HT-treated K14-CreERtam/LSL-K-rasG12D/Ppp6cflox/flox mice developed lip tumors. Microscopic analysis of lip tumor tissue from the mouse indicated hyperkeratotic papillomatous proliferative lesions. It is noteworthy that a region of squamous cell carcinoma in situ (CIS) sacrificed between days 16 to 20. To examine lip epidermis growth, we measured tissue thickness at 4, 8 and 12 days after 4HT injection. The ratio of lip thickness of K14-CreERtam/LSL-K-rasG12D/Ppp6cflox/flox to that of K14-CreERtam/LSL-K-rasG12D mice was 2.3, 24 and 42, at days 4, 8 and 12, respectively.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Loss of protein phosphatase 6 in mouse keratinocytes enhances K-rasG12D-driven tumor promotion.2018
Author(s)
Kurosawa K, Inoue Y, Kakugawa Y, Yamashita Y, Kanazawa K, Kishimoto K, Nomura M, Momoi Y, Sato I, Chiba N, Suzuki M, Ogoh H, Yamada H, Miura K, Watanabe T, Tanuma N, Tachi M, and Shima H
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Journal Title
Cancer Science
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.2018
Author(s)
M Morita, T Sato, M Nomura, Y Sakamoto, Y Inoue, R Tanaka, S Ito, K Kurosawa, K Yamaguchi, Y Sugiura, H Takizaki, Y Yamashita, R Katakura, I Sato, Y Okada, H Watanabe, G Kondoh, et al.
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Journal Title
Cancer Cell
Volume: 12
Pages: 355-367
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ex vivo model of non-small cell lung cancer using mouse lung epithelial cells.2017
Author(s)
Sato T, Morita M, Tanaka R, Inoue Y, Nomura M, Sakamoto Y, Miura K, Ito S, Sato I, Tanaka N, Abe J, Takahashi S, Kawai M, Sato M, Hippo Y, Shima H, Okada Y, Tanuma N
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Journal Title
Oncol Lett
Volume: 14(6)
Pages: 6863-6868
DOI
Related Report
Peer Reviewed / Open Access
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