Development of a Simultaneous fluorescence microscope and PET Imaging System for Awake Mice
| Project/Area Number |
16K19875
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
Takuwa Hiroyuki 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 脳機能イメージング研究部, 研究員(任常) (40508347)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | PET / 蛍光顕微鏡 / 生体脳イメージング / マルチモーダルイメージング / 画像補正 / マルチスケールイメージング / ＰＥＴ / 二光子顕微鏡 / 小動物PET / マルチモダルイメージング / 認知症モデル動物 / 実験装置開発 / 神経科学 / 脳神経疾患 / 放射線 / 応用光学・量子光工学 |
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| Outline of Final Research Achievements |
In this study, we developed a system for simultaneous optical and PET imaging of awake mice. The key element is an open PET scanner (Open PET), which allows it to be combined with another device. A custom made florescence microscope was placed inside the open space of the open PET. The apparatus successfully simultaneous measured cerebral blood flow (CBF) and radioactivity concentration with 11C-raclopride PET.
In order to perform simultaneous FDG-PET measurement and flavoprotein autofluorescence imaging (FAI), we developed a method to correct for the effect of CBF on FAI. FAI is generally weak during changes of blood flow because hemoglobin absorbs fluorescent light. However, our correction method ignores the effects of CBF on FAI during neural activation.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Normative data of dopaminergic neurotransmission functions in substantia nigra measured with MRI and PET: Neuromelanin, dopamine synthesis, dopamine transporters, and dopamine D2 receptors2017
Author(s)
Ito H, Kawaguchi H, Kodaka F, Takuwa H, Ikoma Y, Shimada H, Kimura Y, Seki C, Kubo H, Ishii S, Takano H, Suhara T
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Journal Title
Neuroimage
Volume: 158
Pages: 12-17
DOI
Related Report
Peer Reviewed
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[Journal Article] Changes in effective diffusivity for oxygen during neural activation and deactivation estimated from capillary diameter measured by two-photon laser microscope.2017
Author(s)
Ito H, Takuwa H, Tajima Y, Kawaguchi H, Urushihata T, Taniguchi J, Ikoma Y, Seki C, Ibaraki M, Masamoto K, Kanno I.
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Journal Title
J Physiol Sci.
Volume: 67
Issue: 2
Pages: 325-330
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] In vivo brain imaging for studying possible linkage between pathological hallmark of tauopathy and neuronal loss2017
Author(s)
Hiroyuki Takuwa, Ai Ishikawa, Takuya Urushihata, Takeharu Minamihisamatsu, Masaki Tokunaga, Masafumi Shimojo, Shouko Uchida, Izumi Matsumoto, Ming-Rong Zhang, Tetsuya Suhara, and Makoto Higuchi, Naruhiko Sahara
Organizer
Brain & Brain PET 2017
Place of Presentation
ドイツ ベルリン
Year and Date
2017-04-01
Related Report
Int'l Joint Research
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