Exosomal microRNA transfer regulates cell proliferation and tumor progression in pancreatic cancer

• Project/Area Number: 16K19915
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: NOJIMA HIROYUKI 千葉大学, 医学部附属病院, 助教 (10507320)
• Research Collaborator: Takahashi Makoto ; Nakata Shinichiro
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エクソソーム / 膵癌 / miRNA

Outline of Final Research Achievements

We evaluated exosomes isolated from pancreatic cancer-derived cells. CD 63 was highly expressed in BxPC-3、CFPAC-1、Panc-1 cells, on the other hand CD 63 was slightly expressed in MIA PaCa-2、AsPC-1 cells. These results suggest that exosome production is depended on pancreatic cancer-derived cell.Furthermore, we detected exsomal RNA form pancreatic cancer-derived cells as well as serum form pancreatic cancer patients. We could not detect specific miRNA from various exosomal RNA.

Research Products

• [Journal Article] Duffy antigen receptor for chemokines (DARC) expressing in cancer cells inhibits tumor progression by suppressing CXCR2 signaling in human pancreatic ductal adenocarcinoma. (2017)
  • Author(s): 32.Maeda S, Kuboki S, Nojima H, Shimizu H, Yoshitomi H, Furukawa K, Miyazaki M, Ohtsuka M.
  • Journal Title: Cytokine Volume: 95 Pages: 15-21
  • DOI: 10.1016/j.cyto.2017.02.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Chemokine Receptors, CXCR1 and CXCR2, Differentially Regulate Exosome Release in Hepatocytes (2016)
  • Author(s): Nojima H, Konishi T, Freeman CM, Schuster RM, Japtok L, Kleuser B, Edwards MJ, Gulbins E1, Lentsch AB
  • Journal Title: PLoS One Volume: 11 Issue: 8 Pages: e0161443-e0161443
  • DOI: 10.1371/journal.pone.0161443
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 膵癌における術前FDG-PET検査による細胞増殖能評価，予後予測での有用性 (2017)
  • Author(s): 高橋誠 野島 広之 久保木 知 鈴木 崇之 鈴木 謙介 西野 仁惠 米浦 直子 清水 宏明 吉富 秀幸 古川 勝規 高屋敷 吏 高野 重紹 鈴木 大亮 酒井 望 賀川 真吾 宮崎 勝 大塚 将之
  • Organizer: 第117回 日本外科学会定期学術集会
• [Presentation] 80歳以上の高齢者に対する膵頭十二指腸切除術における手術リスクの検討 (2017)
  • Author(s): 野島広之、久保木知、高屋敷吏 、吉富秀幸、古川勝規 、高屋敷吏、高野重紹 、鈴木大亮、酒井 望 、賀川真吾、宮崎 勝、大塚将之
  • Organizer: 第117回 日本外科学会定期学術集会
• [Presentation] Management and risk factors for postoperative pancreatic fistula grade C in pancreaticoduodenectomy (2017)
  • Author(s): Hiroyuki Nojima, Satoshi Kuboki, Hideyuki Yoshitomi, Katsunori Furukawa, Tsukasa Takayashiki, Shigetsugu Takano, Daisuke Suzuki, Nozomu Sakai, Shingo Kagawa, Masaru Miyazaki, Masayuki Ohtsuka
  • Organizer: Joint Congress of The 6th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association & The 29th Meeting of Japanese Society of Hepato-Biliary-Pancreatic Surgery
• [Presentation] Chemokine receptors, CXCR1 and CXCR2, differentially regulate exosome release in hepatocytes (2016)
  • Author(s): HIROYUKI NOJIMA
  • Organizer: AASLD
  • Place of Presentation: Boston, USA
  • Int'l Joint Research