Establishment of culture and drug sensitivity test system of circulating tumor cell by biomaterial coating

Research Project

Project/Area Number	16K19925
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Digestive surgery
Research Institution	Osaka University
Principal Investigator	MIYAKE Yuichiro 大阪大学, 医学系研究科, 招へい研究員 (80724260)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	末梢循環癌細胞 / シングルセル / 遺伝子変異 / CTC / EMT / 大腸癌 / 血液循環癌細胞 / 単細胞遺伝子解析 / 単細胞培養 / EpCAM
Outline of Final Research Achievements	In this study, we have challenged the development of single cell gene analysis of circulating tumor cell by using dielectrophoresis. As a result of spike test using colon cancer cell lines, we could collect approximately 75% cancer cells that were mixed in the blood sample. Gene mutations were also detected at the single cell level in both CK-positive and CK-negative CTCs of the patients with colorectal cancer. These findings indicate that detection system using the EpCAM antibody has limitation to collect up all CTCs.

Report

(3 results)