Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Outline of Final Research Achievements |
In this study, we have challenged the development of single cell gene analysis of circulating tumor cell by using dielectrophoresis. As a result of spike test using colon cancer cell lines, we could collect approximately 75% cancer cells that were mixed in the blood sample. Gene mutations were also detected at the single cell level in both CK-positive and CK-negative CTCs of the patients with colorectal cancer. These findings indicate that detection system using the EpCAM antibody has limitation to collect up all CTCs.
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