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Establishment of culture and drug sensitivity test system of circulating tumor cell by biomaterial coating

Research Project

Project/Area Number 16K19925
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionOsaka University

Principal Investigator

MIYAKE Yuichiro  大阪大学, 医学系研究科, 招へい研究員 (80724260)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords末梢循環癌細胞 / シングルセル / 遺伝子変異 / CTC / EMT / 大腸癌 / 血液循環癌細胞 / 単細胞遺伝子解析 / 単細胞培養 / EpCAM
Outline of Final Research Achievements

In this study, we have challenged the development of single cell gene analysis of circulating tumor cell by using dielectrophoresis. As a result of spike test using colon cancer cell lines, we could collect approximately 75% cancer cells that were mixed in the blood sample. Gene mutations were also detected at the single cell level in both CK-positive and CK-negative CTCs of the patients with colorectal cancer. These findings indicate that detection system using the EpCAM antibody has limitation to collect up all CTCs.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (1 results)

All 2017

All Presentation (1 results)

  • [Presentation] 大腸癌患者における血液循環癌細胞の単細胞遺伝子変異解析の検討2017

    • Author(s)
      三宅祐一朗、山本浩文、高橋秀和、原口直紹、西村潤一、畑泰司、松田宙、水島恒和、土岐祐一郎、森正樹
    • Organizer
      第72回日本消化器外科学会総会
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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