Combination immunotherapy targeting for chemoresistnat cancer stem cells with PD-L1 expression
| Project/Area Number |
16K19927
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
URAKAWA SHINYA 大阪大学, 医学部附属病院, 医員 (40768975)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 食道癌 / 癌幹細胞 / PD-L1 / 抗腫瘍免疫回避機構 / PD-1 / PDL1 / 幹細胞 / 抗腫瘍免疫 / 治療抵抗性 |
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| Outline of Final Research Achievements |
Although immunohistochemistry showed tumorous PD-L1 expression was associated with poor overall survival in esophageal cancer patients who underwent esophagectomy, no relationship was identified between PD-L1 expression and presence of preoperative therapy or tumor response. In flow cytometry analysis of tissue-infiltrating lymphocytes extracted from pre-therapeutic biopsy tissue and resected specimen, percentage of positive PD-1 in CD8+ cells was higher in non-responders compared with responders while some cases showed increased PD-1-expression after chemotherapy, thus indicating that PD-L1/PD-1 pathways was associated with chemoresistance in esophageal cancer. Now, we are analyzing PD-L1 expression in pre-therapeutic biopsy tissue by immunohistochemistry and establishing a new method to culture tumor tissue together with immune cells to investigate the mechanism of cancer stem cells with PD-L1-expression resistant to chemotherapy.
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Report
(3 results)
Research Products
(4 results)
