| Project/Area Number |
16K19933
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 食道幹細胞 / 食道再生 / iPS細胞 / 遺伝子解析 |
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| Outline of Final Research Achievements |
We proposed regeneration of whole esophageal layers with tissue engineering, adopting with murine esophageal tissue stem cell for the cell sauce and the decellularized esophagus of rat for regeneration scaffold. We could establish the procedure to isolate murine esophageal tissue stem cell through sorting with CD34 positive cell by FACS. And we could decellularize the esophagus of rat in accordance with previous report. We seeded and cultivated isolated esophageal stem cell into decellularized esophagus of rat and confirmed microscopically development of squamous epithelium like cell layer in esophageal scaffold. We established the subcutaneous implant model of rat, but the implanted graft was collapsed and replaced to stringy fibrous tissue. We could observe partially multilayered squamous cell layer, but finally we could not regenerate whole esophageal layers.
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| Academic Significance and Societal Importance of the Research Achievements |
食道疾患の手術では，胃や腸を用いて食道を再建する必要があるが，再生医療の技術により自分の細胞から食道を再生することができれば，健康な臓器を犠牲にする必要がなく，理想的である．今回，食道再生の細胞源となる食道幹細胞をマウス食道から単離し，細胞を洗い流した食道の骨格に移植することで，食道再生することができるかどうかを検証した．結果的に一部の粘膜層は再生されたが、食道の筋肉や血管までは再生できなかった．
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