Comprehensive analysis of DNA hypo methylation in Barrett's esophageal carcinoma

• Project/Area Number: 16K19944
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: HARADA Kazuto 熊本大学, 医学部附属病院, 非常勤診療医師 (70608869)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 食道腺癌 / Barrett食道 / LINE-1 / DNAメチル化 / 食道腺癌部 / 正常部 / 食道癌 / バッレト食道癌

Outline of Final Research Achievements

The level of long interspersed nucleotide element-1 (LINE-1) methylation has become regarded as a surrogate marker of global DNA methylation.　LINE-1 methylation level was evaluated by using Bisulfite-Pyrosequencing method from frozen biopsy sample in esophageal adenocarcinoma, barrett’s esophagus and normal esophageal mucosa. We found that LINE-1 methylation level in esophageal adenocarcinoma was significantly lower than in Barrett;s esophagus and normal mucosa. Lower LINE-1 methylation group showed significantly poor prognosis compared with higher LINE-1 methylation group.

Research Products

• [Journal Article] Prognostic value of LINE-1 methylation level in 321 patients with primary liver cancer including hepatocellular carcinoma and intrahepatic cholangiocarcinoma. (2018)
  • Author(s): Miyata T, Yamashita Y, Baba Y, Harada K, Yamao T, Umezaki N, Tsukamoto M, Kitano Y, Yamamura K, Arima K, Nakagawa S, Okabe H, Imai K, Hashimoto D, Chikamoto A, Shimokawa M, Baba H
  • Journal Title: Oncotarget Volume: 9 Issue: 29 Pages: 20795-20806
  • DOI: 10.18632/oncotarget.25124
  • Peer Reviewed / Open Access
• [Journal Article] LINE-1 methylation level and prognosis in pancreas cancer: pyrosequencing technology and literature review. (2017)
  • Author(s): Yamamura K, Kosumi K, Baba Y, Harada K, Gao F, Zhang X, Zhou L, Kitano Y, Arima K, Kaida T, Takeyama H, Higashi T, Imai K, Hashimoto D, Chikamoto A, Tan X, Baba H
  • Journal Title: Surg Today Volume: 47(12) Issue: 12 Pages: 1450-1459
  • DOI: 10.1007/s00595-017-1539-1
  • Peer Reviewed
• [Presentation] 栄養指標CONUTスコアは食道癌手術後の独立した予後因子である (2016)
  • Author(s): 原田和人、吉田直矢、馬場祥史、志垣博信、岩槻政晃、藏重淳二、小澄敬祐、坂本快郎、宮本裕士、徳永竜馬、辛島龍一、渡邊雅之、馬場秀夫
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: リーガロイヤルホテル大阪（大阪府）
• [Presentation] The relationship between UHRF1 overexpression and LINE-1 hypomethylation in esophageal squamous cell carcinoma (2016)
  • Author(s): Nakamura K、Baba Y, Kosumi K, Harada K, Shigaki H, Miyake K, Kurashige J, Ishimoto T, Iwatsuki M, Yoshida N, Baba H
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: ニューオリンズ（アメリカ）
  • Int'l Joint Research