Exploitation of a liquid biopsy system using circulating tumor DNA in esophageal cancer patients

• Project/Area Number: 16K19952
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Iwate Medical University
• Principal Investigator: Endo Fumitaka 岩手医科大学, 医学部, 助教 (70714442)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: circulating tumor DNA / circulating free DNA / 食道癌 / digital PCR / 次世代シーケンサー / Circulating tumor DNA / 遺伝子パネル / 変異 / シークエンス / 血中遊離DNA / 外科 / 癌 / 遺伝子

Outline of Final Research Achievements

In this study, we performed and established tumor burden monitoring with ctDNA for esophageal squamous cell carcinoma patients treated with either surgery or chemotherapy, using next generation sequencer for mutation detection in primary tumors and digital PCR for ctDNA monitoring in blood samples.
And combinatory procedure of BCT and CPT would be one of the most practical approaches for collection and storage of samples collected during extended follow-up.

Research Products

• [Journal Article] Investigation of operative outcomes of thoracoscopic esophagectomy after triplet chemotherapy with docetaxel, cisplatin, and 5-fluorouracil for advanced esophageal squamous cell carcinoma (2017)
  • Author(s): Akiyama Yuji、Iwaya Takeshi、Endo Fumitaka、Chiba Takehiro、Takahara Takeshi、Otsuka Koki、Nitta Hiroyuki、Koeda Keisuke、Mizuno Masaru、Kimura Yusuke、Sasaki Akira
  • Journal Title: Surgical Endoscopy Volume: 32 Issue: 1 Pages: 391-399
  • DOI: 10.1007/s00464-017-5688-5
  • Peer Reviewed / Open Access
• [Journal Article] Effectiveness of intervention with a perioperative multidisciplinary support team for radical esophagectomy (2017)
  • Author(s): Akiyama Yuji、Iwaya Takeshi、Endo Fumitaka、Shioi Yoshihiro、Kumagai Motoi、Takahara Takeshi、Otsuka Koki、Nitta Hiroyuki、Koeda Keisuke、Mizuno Masaru、Kimura Yusuke、Suzuki Kenji、Sasaki Akira
  • Journal Title: Supportive Care in Cancer Volume: 25 Issue: 12 Pages: 3733-3739
  • DOI: 10.1007/s00520-017-3801-x
  • Peer Reviewed / Open Access
• [Journal Article] Stability of cervical esophagogastrostomy via hand-sewn anastomosis after esophagectomy for esophageal cancer (2017)
  • Author(s): Akiyama Y.、Iwaya T.、Endo F.、Shioi Y.、Chiba T.、Takahara T.、Otsuka K.、Nitta H.、Koeda K.、Mizuno M.、Kimura Y.、Sasaki A.
  • Journal Title: Diseases of the Esophagus Volume: 30 Issue: 5 Pages: 1-7
  • DOI: 10.1093/dote/dow007
  • Peer Reviewed
• [Journal Article] Peripheral vein infusions of amino acids facilitate recovery after esophagectomy for esophageal cancer: Retrospective cohort analysis. (2017)
  • Author(s): Konosu M, Iwaya T, Kimura Y, Akiyama Y, Shioi Y, Endo F, Nitta H, Otsuka K, Koeda K, Sasaki A.
  • Journal Title: Ann Med Surg Volume: 16 (14) Pages: 29-35
  • DOI: 10.1016/j.amsu.2017.01.016
  • Peer Reviewed
• [Journal Article] Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. (2017)
  • Author(s): Iwaya T, Sawada G, Amano S, Kume K, Ito C, Endo F, Konosu M, Shioi Y, Akiyama Y, Takahara T, Otsuka K, Nitta H, Koeda K, Mizuno M, Nishizuka S, Sasaki A, Mimori K.
  • Journal Title: Int J Oncol. Volume: 50(2) Issue: 2 Pages: 441-447
  • DOI: 10.3892/ijo.2016.3817
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 食道癌患者におけるCirculating tumor DNAモニタリングシステムの検討 (2018)
  • Author(s): 岩谷 岳、遠藤 史隆、秋山 有史、千葉 丈広、馬場 誠朗、梅邑 晃、二階 春香、佐藤 慧、八重樫 瑞典、川岸 涼子、松尾 鉄 平、木村 聡元、高原 武志、大塚 幸喜、新田 浩幸、水野 大、肥田 圭介、西塚 哲、佐々木 章
  • Organizer: 第118回日本外科学会学術集会
• [Presentation] 食道扁平上皮癌治療経過における血液腫瘍マーカーの有用性の検討 (2017)
  • Author(s): 遠藤史隆、岩谷岳
  • Organizer: 第117回日本外科学会
  • Place of Presentation: 横浜
  • Year and Date: 2017-04-27
• [Presentation] Evaluation of the utilization of blood collection tubes for cell-free DNA research (2017)
  • Author(s): Fumitaka Endo, Takeshi Iwaya
  • Organizer: アメリカ癌学会2017
  • Place of Presentation: ワシントン
  • Year and Date: 2017-04-01
  • Int'l Joint Research
• [Presentation] Evaluation of the utilization of blood collection tubes for cell-free DNA research (2017)
  • Author(s): Fumitaka Endo, Takeshi Iwaya, Takehiro Chiba, Mizunori Yaegashi, Kohei Kume, Kei Sato, Atsuhiro Arisue, Yutaka Nishinari, Ryoko Kawagishi, Takenori Segawa, Satoshi Nishizuka, Akira Sasaki
  • Organizer: アメリカ癌学会
  • Int'l Joint Research
• [Presentation] 食道扁平上皮癌治療経過における血液腫瘍マーカーの有用性の検討 (2017)
  • Author(s): 遠藤 史隆、岩谷 岳、秋山 有史、塩井 義裕、天野 総、高原 武志、西塚 哲、新田 浩幸、大塚 幸喜、肥田 圭介、水野 大、佐々木 章
  • Organizer: 第117回日本外科学会定期学術総会
• [Presentation] 遺伝子変異情報を用いた食道扁平上皮癌診療システムの開発 (2017)
  • Author(s): 遠藤 史隆、岩谷 岳、秋山 有史、梅邑 晃、二階 春香、鳥谷 洋右、高原 武志、新田 浩幸、大塚 幸喜、肥田 圭介、水野 大、松本 主之、西塚 哲、佐々木 章
  • Organizer: JDDW（日本消化器関連学会週間）第59回消化器病学会大会
• [Presentation] Exploitation of a liquid biopsy system using circulating tumor DNA in esophageal cancer patients (2017)
  • Author(s): Takeshi Iwaya, Fumitaka Endo, Yasushi Sasaki, Mizunori Yaegashi, Takehiro Chiba, Yuji Akiyama, Mari Masuda, Tesshi Yamada, Takashi Tokino, Satoshi Nishizuka
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 食道扁平上皮癌パネルを用いた変異解析の有用性の検討 (2017)
  • Author(s): 岩谷 岳、遠藤 史隆、西塚 哲、秋山 有史、高原 武志、大塚 幸喜、新田 浩幸、肥田 圭介、水野 大、佐々木 章
  • Organizer: 第72回消化器外科学会総会
• [Presentation] Mutation detection by target sequence analyses using tissue-specific panels in esophageal squamous cell carcinoma (2017)
  • Author(s): Takeshi Iwaya, Fumitaka Endo, Kohei Kume, Yasushi Sasaki, Takashi Tokino, Satoshi Nishizuka
  • Organizer: アメリカ癌学会
  • Int'l Joint Research