Basic research of Heat Shock Protein 27 as a new target to overcome 5-fluorouracil resistance.

Research Project

Project/Area Number	16K19953
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Digestive surgery
Research Institution	Keio University
Principal Investigator	ASADA YUSUKE 慶應義塾大学, 医学部(信濃町), 助教 (50649952)
Research Collaborator	TSURUTA Masashi
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	大腸癌 / 化学療法 / 耐性 / 5-fluorouracil / Heat Shock Protein 27 / 5-Fluorouracil / Heat shock protein 27 / 5-FU / HSP27 / 癌 / 薬剤反応性 / 臨床
Outline of Final Research Achievements	We generated the drug-resistant subclone of human colon cancer cell line WiDr by exposure to 5-fluorouracil (5-FU). Higher Heat Shock Protein 27 (HSP27) level was demonstrated in drug-resistant subclone than in naïve cell line. Down-regulation of overexpressed HSP27 suppressed generated 5-FU resistance in drug-resistant subclone. These data is important to develop a new therapy targeted on HSP27, especially to overcome generated resistance during chemotherapy in colon cancer.

Report

(3 results)