| Project/Area Number |
16K19959
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Maeda Masahiro 国立研究開発法人国立がん研究センター, 研究所, 研究員 (30738703)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | がん関連線維芽細胞 / H3K27me3 / WNT5A / エピジェネティクス / ヒストン修飾 |
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| Outline of Final Research Achievements |
Cancer-associated fibroblasts (CAFs), a major cellular component of cancer stroma, play a crucial role in cancer progression. In this study, we aimed to identify epigenetic characteristics of CAFs, which may underlie stable tumor-promoting capacities of CAFs. Primary culture of CAFs and non-CAFs was established from gastric surgical specimens. Focusing on H3K27me3, a repressive histone mark that is stable and essential for cell-specific differentiation, we revealed that CAFs had genome-wide alterations of H3K27me3, leading to aberrant production of various tumor promoting factors due to decreased H3K27me3. Among them, we identified WNT5A as a therapeutic target derived from CAFs. This study suggested that epigenetic alterations may determine CAF's biological behaviors.
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