Mechanism of intimal hyperplasia development at valve site of human vein graft
| Project/Area Number |
16K19962
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cardiovascular surgery
|
|---|
| Research Institution | Asahikawa Medical College |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 内膜肥厚 / 静脈グラフト / 下肢動脈疾患 / 弁部 / グラフト狭窄 / 末梢動脈疾患 / 下肢動脈バイパス / 静脈弁 / 自家静脈グラフト / 大伏在静脈 / 弁部内膜肥厚 / 閉塞性動脈硬化症 / 外科的血行再建 |
|---|
| Outline of Final Research Achievements |
Venous valves are essential, but are prone to injury, thrombosis and fibrosis. We compared the behavior and gene expression of smooth muscle cells (SMCs) in the valve sinus vs non-valve sites to elucidate biological differences associated with vein valves. Tissue explants of fresh human saphenous veins were prepared, and the migration ofSMCs from explants of valve sinus vs non-valve sinus areas was measured. Valve SMCs demonstrated greater proliferation in tissue explants compared to non-valve SMCs. Valve SMCs also proliferated faster than non-valve SMCs in response to PDGF-BB.PDGF-FGF co-activation system caused the difference between both SMCs.This mechanism was applied to the ex-vivo, but no difference was seen cell functions between both SMCs.We finally tried gene screenign using RNA sequence, demonstrating that 37 genes were differentially expressed by valvec compared to non-valve tissue (11 veins).
|
Report
(3 results)
Research Products
(4 results)
