Fabrication of scaffold-free cardiac construct by using bio 3D printer and analysis of function

• Project/Area Number: 16K19968
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Saga University
• Principal Investigator: Arai Kenichi 佐賀大学, 医学部, 寄附講座助教 (40752960)
• Research Collaborator: Nakayama Kochi ; Kobayashi Eiji
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: バイオ3Dプリンタ / ヒトiPS細胞由来心筋細胞 / 心筋ポンプ / 組織工学 / 再生医療 / スフェロイド / スキャフォールドフリー / 心筋組織工学 / iPS細胞 / 心筋疾患外科学 / 再生医学

Outline of Final Research Achievements

Firstly, we optimized cardiac spheroids by mixing human iPS derived cardiomyocytes, endothelial cell, and fibroblasts. This result indicates that the addition of fibroblasts and endothelial cells promotes rapid cell self-organization and enhances cardiac spheroid stability with regards to shape and size. Secondly, we fabricated cardiac constructs by using the bio-3D printer. Cardiac constructs were cultured on the needle arrays for 7 days. Beating of the fused constructs was observed. After removal from the needle array, we confirmed electrical stimulation response of constructs, micro-vascular formation and the rearrangements of cardiomyocytes inside the construct.
In future, we will transplant this tubular cardiac construct to develop a new cardiac regenerative therapy.

Research Products

• [Presentation] バイオ3Dプリンタを用いて作製した心筋組織体の薬理応答の検討 (2018)
  • Author(s): 荒井健一、中山功一
  • Organizer: 第17回日本再生医療学会総会
• [Presentation] バイオ3Dプリンタを用いたヒトiPS細胞由来心筋組織構造体の作製 (2018)
  • Author(s): 遠山周吾、荒井健一、川口新治、木村成卓、藤田淳、福田恵一、中山功一、小林英司
  • Organizer: 第17回日本再生医療学会総会
  • Invited
• [Presentation] バイオ3Dプリンタを用いたチューブ型心筋構造体の作製 (2017)
  • Author(s): 荒井健一、小島敦子、迎洋輔、伊藤学、森田茂樹、中山功一
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-07
• [Presentation] Fabrication of scaffold-free cardiac construct by bio 3D-printer. (2016)
  • Author(s): Arai K, Ojima A, Mukae Y, Morita S, Nakayama K.
  • Organizer: International Conference on Biofabrication
  • Place of Presentation: Wiston-Salem, North Carolina, USA
  • Year and Date: 2016-10-29
  • Int'l Joint Research
• [Presentation] Addition of human iPS-derived neural progenitors influences the contractile function of cardiac spheroids (2016)
  • Author(s): Mukae Y, Itoh M, Furukawa K, Kitsuka T, Arai K, Oyama J, Nakayama K, Node K, Morita S
  • Organizer: International Conference on Biofabrication
  • Place of Presentation: Wiston-Salem, North Carolina, USA
  • Year and Date: 2016-10-29
  • Int'l Joint Research
• [Book] バイオ・医療への3Dプリンティング技術の開発最前線「バイオ3Dプリンタを用いた立体構造体の作製と将来的な展望」 (2016)
  • Author(s): 荒井健一、中山功一
  • Total Pages: 230
  • Publisher: シーエムシー出版