| Project/Area Number |
16K19968
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Saga University |
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Principal Investigator |
Arai Kenichi 佐賀大学, 医学部, 寄附講座助教 (40752960)
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| Research Collaborator |
Nakayama Kochi
Kobayashi Eiji
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | バイオ3Dプリンタ / ヒトiPS細胞由来心筋細胞 / 心筋ポンプ / 組織工学 / 再生医療 / スフェロイド / スキャフォールドフリー / 心筋組織工学 / iPS細胞 / 心筋疾患外科学 / 再生医学 |
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| Outline of Final Research Achievements |
Firstly, we optimized cardiac spheroids by mixing human iPS derived cardiomyocytes, endothelial cell, and fibroblasts. This result indicates that the addition of fibroblasts and endothelial cells promotes rapid cell self-organization and enhances cardiac spheroid stability with regards to shape and size. Secondly, we fabricated cardiac constructs by using the bio-3D printer. Cardiac constructs were cultured on the needle arrays for 7 days. Beating of the fused constructs was observed. After removal from the needle array, we confirmed electrical stimulation response of constructs, micro-vascular formation and the rearrangements of cardiomyocytes inside the construct.
In future, we will transplant this tubular cardiac construct to develop a new cardiac regenerative therapy.
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