Investigation into the effects of photodynamic therapy using an interstitial pneumonia animal model

• Project/Area Number: 16K19985
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: IMAI KENTARO 東京医科大学, 医学部, 助教 (30408140)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 肺癌 / 光線力学的治療 / 間質性肺炎 / 末梢 / 小型

Outline of Final Research Achievements

We developed a bleomycin-induced disorder model using rats, and performed thoracotomy under general anesthesia and laser irradiation of the lungs. The test groups included bleomycin-administered and bleomycin-nonadministered groups. HE histopathological findings showed fibroblast proliferation and pleural fibrosis in the bleomycin-administered group, confirming lung fibrosis. In the laser irradiation site, neutrophilia, fibrin leaching, and hypertrophy of the alveolar epithelium were confirmed, and tissue necrosis was observed. Laser irradiation showed no effect on the tissue around the laser-irradiated portion. Even in the bleomycin-nonadministered group, tissue necrosis of the laser-irradiated site was observed, indicating the laser irradiation effect of PDT. In the tissues around the laser irradiation site, findings suggesting inflammation were not evident. Herein, the effects of PDT on the rat lung interstitium were negligible in the bleomycin-induced disorder model.