Effect of pentraxin 3 in subacute phase of ischemic stroke

• Project/Area Number: 16K20005
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Mie University
• Principal Investigator: Shindo Akihiro 三重大学, 医学部附属病院, 助教 (60422820)
• Research Collaborator: TOMIMOTO Hidekazu 三重大学, 大学院医学系研究科 神経病態内科学, 教授 ; ARAI Ken マサチューセッツ総合病院
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脳卒中 / 脳梗塞 / 炎症 / 血液脳関門 / 血管新生 / ペントラキシン３ / オリゴデンドロサイト / 白質 / ペントラキシン3 / 神経再生

Outline of Final Research Achievements

The concept of the neurovascular unit is widely accepted nowadays, since proposed as a conceptual framework for investigating the mechanisms of both blood vessels and brain cells, such as neurons, astrocytes and oligodendrocytes.Pentraxin 3 (PTX3) upregulates during cerebrovascular diseases.In this study, we examined the hypothesis that PTX3 may have important roles after ischemic stroke in and sub-acute phase, mediate crosstalk between reactive astrocytes and endothelial cells and other types of cells. We added rPTX3 to RBE.4 cells and examined the angiogenic response using in vitro angiogenesis assay. Treatment with rPTX3 might suppress the number of vessel-like structures in vitro. Next,we used siRNA to make the suppression of PTX3 after stroke.Downregulation of PTX3 in sub-acute phase may increase angiogenesis.

Research Products

• [Journal Article] Astrocyte-Derived Pentraxin 3 Supports Blood-Brain Barrier Integrity Under Acute Phase of Stroke (2016)
  • Author(s): Shindo A, Maki T, Mandeville ET, Liang AC, Egawa N, Itoh K, Itoh N, Borlongan M, Holder JC, Chuang TT, McNeish JD, Tomimoto H, Lok J, Lo EH, Arai K.
  • Journal Title: Stroke Volume: 47 Issue: 4 Pages: 1094-100
  • DOI: 10.1161/strokeaha.115.012133
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of Aging on Neural Stem/Progenitor Cells and Oligodendrocyte Precursor Cells After Focal Cerebral Ischemia in Spontaneously Hypertensive Rats. (2016)
  • Author(s): Liang AC, Mandeville ET, Maki T, Shindo A, Som AT, Egawa N, Itoh K, Chuang TT, McNeish JD, Holder JC, Lok J, Lo EH, Arai K.
  • Journal Title: Cell Transplant Volume: 25 Issue: 4 Pages: 705-714
  • DOI: 10.3727/096368916x690557
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Magnesium sulfate protects oligodendrocyte lineage cells in a rat cell-culture model of hypoxic-ischemic injury. (2016)
  • Author(s): Itoh K, Maki T, Shindo A, Egawa N, Liang AC, Itoh N, Lo EH, Lok J, Arai K.
  • Journal Title: Neurosci Res Volume: 106 Pages: 66-69
  • DOI: 10.1016/j.neures.2015.12.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Critical Care with thiamine treatment in a patient of Marchiafava-Bingami disease (2017)
  • Author(s): A Shindo, R Asada, K Suzuki, H Ishikawa, E Kawamoto, M Fujioka, H Tomimoto, H Imai
  • Organizer: Society of Critical Care Medicine’s (SCCM) 47th Critical Care Congress
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Pentraxin 3 supports blood-brain barrier integrity after ischemic stroke (2017)
  • Author(s): Akihiro Shindo, Takakuni Maki, Naohiro Egawa, Anna C. Liang, Kanako Itoh, Eng H. Lo, Ken Arai, Hidekazu Tomimoto
  • Organizer: World congress of Neurology 2017
  • Int'l Joint Research
• [Presentation] Astrocyte-derived Pentraxin 3 Supports Blood-Brain Barrier Integrity after ischemic stroke (2016)
  • Author(s): A Shindo, T Maki, N Egawa, K Itoh, A Liang, E Lo, K Arai, H Tomimoto
  • Organizer: Asian and Oceanian Congress of Neurology
  • Place of Presentation: KLCC, Kuala Lumpur, Malaysia
  • Year and Date: 2016-08-18
  • Int'l Joint Research
• [Presentation] Astrocytic pentraxin 3 supports blood-brain barrier integrity under acute phase of ischemic stroke (2016)
  • Author(s): A Shindo, T Maki, N Egawa, K Itoh, A Liang, E Lo, K Arai, H Tomimoto
  • Organizer: 日本神経学会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2016-05-18
• [Book] Non-neuronal mechanisms of brain damage and repair after stroke (2016)
  • Author(s): A Shindo, T Maki, K Itoh, N Miyamoto, N Egawa, AC Liang, T Noro, J Lok, EH Lo, K Arai
  • Total Pages: 408
  • Publisher: Springer