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Novel therapeutic approach for glioblastoma with iPS cells-derived CAR-T cells

Research Project

Project/Area Number 16K20022
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurosurgery
Research InstitutionNara Medical University

Principal Investigator

Nakzawa Tsutomu  奈良県立医科大学, 医学部, 研究員 (00772500)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords遺伝子免疫細胞治療 / CAR / 膠芽腫 / iPS細胞 / T細胞
Outline of Final Research Achievements

In this study, we aim to establish EGFRvIII-specific CAR (EvCAR)-expressing iPS cells and differentiate into T cells from the EvCAR-iPS cells. The EvCAR-expressing iPS cells were established by transducing EvCAR gene-carrying lentivirus into iPS cells. Hematopoietic stem cells (HSC) were induced from the EvCAR-iPS cells in the presence of the defined cytokines under the feedeless condition. CD3 positive and EvCAR positive cells were induced from the HSC in the presence of the defined cytokines on OP9Δ1 cells.
In conclusion, we can induce the iPS cell-derived EvCAR-T cells in vitro. The EvCAR-T cells can be a promising anti-tumoral cell source for glioblastoma if their anti-tumor effects for glioblastoma are able to be evaluate.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Minodronic Acid in Combination with γδT Cells Induces Apoptosis of Non-small Cell Lung Carcinoma Cell Lines.2016

    • Author(s)
      Yasukawa M, Nakazawa T, Kawaguchi T, Kawai N, Tsujimura T, Tojo T, Taniguchi S.
    • Journal Title

      Anticancer Research

      Volume: 36 Pages: 5883-5886

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Antitumor effects of minodronate, a third-generation nitrogen-containing bisphosphonate, in synergy with γδT cells in human glioblastoma in vitro and in vivo.2016

    • Author(s)
      Nakazawa T, Nakamura M, Matsuda R, Nishimura F, Park YS, Motoyama Y, Hironaka Y, Nakagawa I, Yokota H, Yamada S, Tamura K, Takeshima Y, Omoto K, Tanaka Y, Ouji Y, Yoshikawa M, Tsujimura T, Nakase H.
    • Journal Title

      J Neurooncol

      Volume: 129 Issue: 2 Pages: 231-241

    • DOI

      10.1007/s11060-016-2186-x

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tumor-Inhibition Effect of Levetiracetam in Combination with Temozolomide in Glioblastoma Cells2016

    • Author(s)
      Marutani A., Nakamura M., Nishimura F., Nakazawa T., Matsuda R., Hironaka Y., Nakagawa I., Tamura K., Takeshima Y., Motoyama Y., Park Y-S, Ouji Y., Yoshikawa M., Nakase H.
    • Journal Title

      Neurochemical Journal

      Volume: Volume10, No4 Issue: 1 Pages: 333-339

    • DOI

      10.1134/s1819712416040073

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Antitumor effects of minodronate, a third-generation nitrogen-containing bisphosphonate, in synergy with γδT cells, in human glioblastoma in vitro and in vivo2018

    • Author(s)
      Tsutomu Nakazawa, Mitsutoshi Nakamura, Ryosuke Matsuda, Fumihiko Nishimura, Young Soo Park, Yasushi Motoyama, Ichiro Nakagawa, Hiroshi Yokota, Shuichi Yamada, Kentaro Tamura, Yasuhiro Takeshima, Kouji Omoto, Yoshitaka Tanaka, Youichi Shida, Toshiharu Murakami, Rieko Takanabe, Takahiro Tsujimura, Hiroyuki Nakase
    • Organizer
      The AACR Special Conference on Immunobiology of Primary and Metastatic CNS Cancer
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高純度NK細胞を用いた膠芽腫細胞に対する抗腫瘍効果とTMZを用いた 併用効果の基礎的検討2017

    • Author(s)
      田中 祥貴, 中澤 務, 中村 光利, 辻村 貴弘, 竹島 靖浩, 松田 良介, 田村 健太郎, 山田 修一, 中川 一郎, 西村 文彦, 横田 浩, 本山 靖, 朴 永銖, 中瀬 裕之
    • Organizer
      第14回日本免疫治療学研究会学術集会
    • Place of Presentation
      東京大学 伊藤国際学術研究センター
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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