| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Cerebral venous ischemia is a rare but potentially significant complication of neurosurgical procedures. However, it is still unclear how intraoperative sacrifice of cerebral veins affects regional neural cell activations: especially endogenous neural stem cell. To elucidate its pathophysiology, we examine this in vivo study. Two cortical veins were occluded photochemically using rose Bengal dye in male Wister rats. BrdU was intermittently administrated intraperitoneally after the venous occlusion for several days. Immunohistochemical examination showed double positive (BrdU+NeuN+) cell was expressed around ischemic core at day 35 after the ischemia, and double positive (BrdU+Dcx+) cell was also expressed around ischemic core at day 15 after the ischemia. However, double positive (BrdU+Dcx+) cell was not observed at the periventricular zone. This study demonstrated new findings of the expression of regional endogenous neural stem cells after cortical venous ischemia.
|
