Diagnosis of glioma by PCR method and identification of malignance factor of glioma

• Project/Area Number: 16K20029
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Fujita Health University
• Principal Investigator: Nakae Shunsuke 藤田保健衛生大学, 医学部, 助教 (20622971)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: グリオーマ / 頭蓋内遠隔再発 / +8q / IDH mutant gliomas / TP53 mutation / 8q gain / high copy number / remote recurrence / major fiber bundles / microarray

Outline of Final Research Achievements

In this study, we classified the recurrence pattern of IDH mutant gliomas into five patterns, and clarified that IDH mutant gliomas with mutation of p53 take remote recurrence and local recurrence. In the case of the intracranial remote recurrence pattern, we showed that intervention of nerve fibers between the initial and recurrent lesions in the image analysis, and an increase of the copy number variations in Q arm of the eighth chromosome in the analysis by the next generation sequencer (especially high copy gain in 8q22-24 region). Based on the above, now we are being investigating the C-MYC and the Wnt signaling pathway.

Research Products

• [Journal Article] Novel Application of Time-Spatial Labeling Inversion Pulse Magnetic Resonance Imaging for Diagnosis of External Hydrocephalus (2018)
  • Author(s): Nakae Shunsuke、Murayama Kazuhiro、Adachi Kazuhide、Kumai Tadashi、Abe Masato、Hirose Yuichi
  • Journal Title: World Neurosurgery Volume: 109 Pages: 197-201
  • DOI: 10.1016/j.wneu.2017.09.175
  • Peer Reviewed
• [Journal Article] Remote intracranial recurrence of <i>IDH</i> mutant gliomas is associated with <i>TP53</i> mutations and an 8q gain (2017)
  • Author(s): Nakae Shunsuke、Kato Takema、Murayama Kazuhiro、Sasaki Hikaru、Abe Masato、Kumon Masanobu、Kumai Tadashi、Yamashiro Kei、Inamasu Joji、Hasegawa Mitsuhiro、Kurahashi Hiroki、Hirose Yuichi
  • Journal Title: Oncotarget Volume: 8 Issue: 49 Pages: 84729-84742
  • DOI: 10.18632/oncotarget.20951
  • Peer Reviewed / Open Access
• [Journal Article] Atypical intraosseous meningioma with growth hormone deficiency and hyperparathyroidism after craniospinal irradiation (2017)
  • Author(s): Nakae Shunsuke、Uchida Hiroki、Takayanagi Takeshi、Hasegawa Mitsuhiro、Hirose Yuichi
  • Journal Title: Child's Nervous System Volume: 33 Issue: 12 Pages: 2077-2078
  • DOI: 10.1007/s00381-017-3587-7
  • Peer Reviewed
• [Journal Article] Prediction of genetic subgroups in adult supra tentorial gliomas by pre- and intraoperative parameters (2017)
  • Author(s): Nakae S, Murayama K, Sasaki H, Kumon M, Nishiyama Y, Ohba S, Adachi K, Nagahisa S, Hayashi T, Inamasu J, Abe M, Hasegawa M, Hirose Y
  • Journal Title: Journal of Neuro-Oncology Volume: 131(2) Issue: 2 Pages: 403-12
  • DOI: 10.1007/s11060-016-2313-8
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Intracranial remote recurrence in IDH mutant gliomas is associated with TP53 mutations and 8q gain (2017)
  • Author(s): Shunsuke Nakae
  • Organizer: ASNO 2017
  • Int'l Joint Research
• [Presentation] IDH変異型グリオーマにおける頭蓋内遠隔転移とTP53変異及び8qgainとの関連性 (2017)
  • Author(s): 中江俊介
  • Organizer: 日本の腫瘍学会学術集会
• [Presentation] IDH変異型グリオーマにおける頭蓋内遠隔転移とTP53変異及び8qgainとの関連性 (2017)
  • Author(s): 中江俊介
  • Organizer: 日本臨床分子形態学会総会・学術集会
• [Presentation] IDH変異型グリオーマの再発パターンとコピー数異常の関連性の検討 (2017)
  • Author(s): 中江俊介
  • Organizer: 第40回日本脳神経CI学会総会
  • Place of Presentation: 鹿児島サンロイヤルホテル