Novel treatment for metastatic bone tumors

• Project/Area Number: 16K20042
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya City University
• Principal Investigator: Miwa Shinji 名古屋市立大学, 大学院医学研究科, 助教 (40753455)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 骨転移 / GSK-3β / 前立腺癌 / 癌 / 新規治療 / 蛍光イメージング / 手術 / 蛍光蛋白 / イメージング

Outline of Final Research Achievements

The human prostate cancer PC-3 cells were genetically engineered to express RFP using a retrovirus. The cells were treated with SB216763 or AR-A014418 and evaluated by clonogenic assay. In the in vitro study, GSK-3β inhibitor inhibited proliferation of PC-3-RFP cells in a dose-dependent manner. PC-3-RFP cells in Matrigel were injected into the intramedullary cavity of the left tibia. One week after intratibial injection of cancer cells, the mice were treated by SB216763 or AR-A014418. GSK-3β inhibitors significantly reduced the fluorescent area of the tumor, tumor volume, and tumor weight.

Research Products

• [Journal Article] Anti-tumor effects of a nonsteroidal anti-inflammatory drug zaltoprofen on chondrosarcoma via activating peroxisome proliferator-activated receptor gamma and suppressing matrix metalloproteinase-2 expression (2018)
  • Author(s): Higuchi T, Takeuchi A, Munesue S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Inatani H, Shimozaki S, Kato T, Aoki Y, Abe K, Taniguchi Y, Aiba H, Murakami H, Harashima A, Yamamoto Y, Tsuchiya H
  • Journal Title: Cancer Med Volume: 印刷中
  • NAID: 120006605139
  • Peer Reviewed / Open Access
• [Journal Article] Imaging DNA Repair After UV Irradiation Damage of Cancer Cells in Gelfoam; Histoculture (2018)
  • Author(s): Miwa S, Hoffman RM
  • Journal Title: Methods Mol Biol Volume: 1760 Pages: 199-203
  • Int'l Joint Research
• [Journal Article] Tumor-targeting Salmonella typhimurium A1-R is a highly effective general therapeutic for undifferentiated soft tissue sarcoma patient-derived orthotopic xenograft nude-mouse models (2018)
  • Author(s): Igarashi K、Kawaguchi K、Kiyuna T、Miyake K、Miyake M、Singh AS、Eckardt MA、Nelson SD、Russell TA、Dry SM、Li Y、Yamamoto N、Hayashi K、Kimura H、Miwa S、Tsuchiya H、Singh SR、Eilber FC、Hoffman RM
  • Journal Title: Biochem Biophys Res Commun Volume: 497 Issue: 4 Pages: 1055-1061
  • DOI: 10.1016/j.bbrc.2018.02.174
  • Peer Reviewed
• [Journal Article] Clinical outcomes of radio-hyperthermo-chemotherapy for soft tissue sarcoma compared to a soft tissue sarcoma registry in Japan: a retrospective matched-pair cohort study (2018)
  • Author(s): Aiba H、Yamada S、Mizutani Jun、Yamamoto Norio、Okamoto Hideki、Hayashi Katsuhiro、Kimura Hiroaki、Takeuchi Akihiko、Miwa Shinji、Kawai Akira、Yoshimura Kenichi、Tsuchiya Hiroyuki、Takanobu Otsuka
  • Journal Title: Cancer Med Volume: 印刷中 Issue: 4 Pages: 1560-1571
  • DOI: 10.1002/cam4.1366
  • Peer Reviewed / Open Access
• [Journal Article] Tumor-targeting Salmonella typhimurium A1-R combined with recombinant methioninase and cisplatinum eradicates an osteosarcoma cisplatinum-resistant lung metastasis in a patient-derived orthotopic xenograft (PDOX) mouse model: Decoy, trap and kill chemotherapy moves toward the clinic (2018)
  • Author(s): Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Li S, Han Q, Tan Y, Zhao M, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM
  • Journal Title: Cell Cycle Volume: 印刷中 Issue: 6 Pages: 801-809
  • DOI: 10.1080/15384101.2018.1431596
  • Peer Reviewed / Open Access
• [Journal Article] Mid- to long-term clinical outcome of giant cell tumor of bone treated with calcium phosphate cement following thorough curettage and phenolization. (2018)
  • Author(s): Takeuchi A, Suwanpramote P, Yamamoto N, Shirai T, Hayashi K, Kimura H, Miwa S, Higuchi T, Abe K, Tsuchiya H.
  • Journal Title: J Surg Oncol. Volume: 印刷中 Issue: 6 Pages: 1232-1238
  • DOI: 10.1002/jso.24971
  • Peer Reviewed
• [Journal Article] Recombinant methioninase in combination with doxorubicin (DOX) overcomes first-line DOX resistance in a patient-derived orthotopic xenograft nude-mouse model of undifferentiated spindle-cell sarcoma (2018)
  • Author(s): Igarashi K, Kawaguchi K, Li S, Han Q, Tan Y, Murakami T, Kiyuna T, Miyake K, Miyake M, Singh AS, Eckardt MA, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Singh SR, Eilber FC, Hoffman RM
  • Journal Title: Cancer Lett Volume: 417 Pages: 168-173
  • DOI: 10.1016/j.canlet.2017.12.028
  • Peer Reviewed
• [Journal Article] The Efficacy of Wide Resection for Musculoskeletal Metastatic Lesions of Renal Cell Carcinoma (2018)
  • Author(s): Higuchi T, Yamamoto N, Hayashi K, Takeuchi A, Kato S, Miwa S, Abe K, Taniguchi Y, Aiba H, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 38 Pages: 577-582
  • Peer Reviewed
• [Journal Article] Growth of doxorubicin-resistant undifferentiated spindle-cell sarcoma PDOX is arrested by metabolic targeting with recombinant methioninase (2018)
  • Author(s): Igarashi K, Li S, Han Q, Tan Y, Kawaguchi K, Murakami T, Kiyuna T, Miyake K, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 119 Issue: 4 Pages: 3537-3544
  • DOI: 10.1002/jcb.26527
  • Peer Reviewed
• [Journal Article] Temozolomide combined with irinotecan regresses a cisplatinum-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) precision-oncology mouse model (2017)
  • Author(s): Igarashi K、Kawaguchi K、Kiyuna T、Miyake K、Miyake M、Li Y、Nelson SD、Dry SM、Singh AS、Elliott IA、Russell TA、Eckardt MA、Yamamoto N、Hayashi K、Kimura H、Miwa S、Tsuchiya H、Eilber FC、Hoffman RM
  • Journal Title: Oncotarget Volume: 9 Issue: 8 Pages: 7774-7781
  • DOI: 10.18632/oncotarget.22892
  • NAID: 120006454991
  • Peer Reviewed / Open Access
• [Journal Article] Current status of immunotherapy for sarcomas (2017)
  • Author(s): Miwa Shinji、Nishida Hideji、Tsuchiya Hiroyuki
  • Journal Title: Immunotherapy Volume: 9 Issue: 16 Pages: 1331-1338
  • DOI: 10.2217/imt-2017-0101
  • NAID: 120006454997
  • Peer Reviewed
• [Journal Article] Risk factors for postoperative deep infection in bone tumors (2017)
  • Author(s): Miwa Shinji、Shirai Toshiharu、Yamamoto Norio、Hayashi Katsuhiro、Takeuchi Akihiko、Tada Kaoru、Kajino Yoshitomo、Inatani Hiroyuki、Higuchi Takashi、Abe Kensaku、Taniguchi Yuta、Tsuchiya Hiroyuki
  • Journal Title: PLoS One Volume: 12 Issue: 11 Pages: e0187438-e0187438
  • DOI: 10.1371/journal.pone.0187438
  • NAID: 120006374163
  • Peer Reviewed / Open Access
• [Journal Article] Temozolomide combined with irinotecan caused regression in an adult pleomorphic rhabdomyosarcoma patient-derived orthotopic xenograft (PDOX) nude-mouse model (2017)
  • Author(s): Igarashi Kentaro、Kawaguchi Kei、Kiyuna Tasuku、Murakami Takashi、Miwa Shinji、Nelson Scott D.、Dry Sarah M.、Li Yunfeng、Singh Arun S.、Kimura Hiroaki、Hayashi Katsuhiro、Yamamoto Norio、Tsuchiya Hiroyuki、Eilber Fritz C.、Hoffman Robert M.
  • Journal Title: Oncotarget Volume: 8 Issue: 44 Pages: 75874-75880
  • DOI: 10.18632/oncotarget.16548
  • Peer Reviewed / Open Access
• [Journal Article] A novel anionic-phosphate-platinum complex effectively targets an undifferentiated pleomorphic sarcoma better than cisplatinum and doxorubicin in a patient-derived orthotopic xenograft (PDOX) (2017)
  • Author(s): Igarashi Kentaro、Kawaguchi Kei、Murakami Takashi、Kiyuna Tasuku、Miyake Kentaro、Yamamoto Norio、Hayashi Katsuhiro、Kimura Hiroaki、Nelson Scott D.、Dry Sarah M.、Li Yunfeng、Singh Arun S.、Miwa Shinji、Odani Akira、Eilber Fritz C.、Tsuchiya Hiroyuki、Hoffman Robert M.
  • Journal Title: Oncotarget Volume: 8 Issue: 38 Pages: 63353-63359
  • DOI: 10.18632/oncotarget.18806
  • Peer Reviewed / Open Access
• [Journal Article] Effective Metabolic Targeting of Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models with Recombinant Methioninase (2017)
  • Author(s): Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Murakami T, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Hoffman RM
  • Journal Title: Anticancer Res Volume: 37 Pages: 4807-4812
  • Peer Reviewed
• [Journal Article] Spontaneous shrinkage of solitary osteochondromas (2017)
  • Author(s): Aiba Hisaki、Yamada Satoshi、Yamamoto Norio、Hayashi Katsuhiro、Miwa Shinji、Tsuchiya Hiroyuki、Otsuka Takanobu
  • Journal Title: Skeletal Radiol Volume: 47 Issue: 1 Pages: 61-68
  • DOI: 10.1007/s00256-017-2760-0
  • Peer Reviewed
• [Journal Article] Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines (2017)
  • Author(s): Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh AS, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM
  • Journal Title: Anticancer Res Volume: 37 Pages: 4081-4084
  • Peer Reviewed
• [Journal Article] Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen (2017)
  • Author(s): Higuchi Takashi、Yamamoto Norio、Nishida Hideji、Hayashi Katsuhiro、Takeuchi Akihiko、Kimura Hiroaki、Miwa Shinji、Inatani Hiroyuki、Shimozaki Shingo、Kato Takashi、Aoki Yu、Abe Kensaku、Taniguchi Yuta、Tsuchiya Hiroyuki
  • Journal Title: Int Orthop Volume: 41 Issue: 10 Pages: 2189-2197
  • DOI: 10.1007/s00264-017-3499-x
  • NAID: 120006343969
  • Peer Reviewed
• [Journal Article] An unusual case of proximal humeral simple bone cyst in an adult from secondary cystic change (2017)
  • Author(s): Rosario Mamer S.、Yamamoto Norio、Hayashi Katsuhiro、Takeuchi Akihiko、Kimura Hiroaki、Miwa Shinji、Higuchi Takashi、Inatani Hiroyuki、Abe Kensaku、Taniguchi Yuta、Aiba Hisaki、Tsuchiya Hiroyuki
  • Journal Title: World J Surg Oncol Volume: 15 Issue: 1 Pages: 102-102
  • DOI: 10.1186/s12957-017-1166-8
  • Peer Reviewed / Open Access
• [Journal Article] Intra-arterial administration of tumor-targeting Salmonella typhimurium A1-R regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model (2017)
  • Author(s): Igarashi Kentaro、Kawaguchi Kei、Murakami Takashi、Kiyuna Tasuku、Miyake Kentaro、Nelson Scott D.、Dry Sarah M.、Li Yunfeng、Yanagawa Jane、Russell Tara A.、Singh Arun S.、Yamamoto Norio、Hayashi Katsuhiro、Kimura Hiroaki、Miwa Shinji、Tsuchiya Hiroyuki、Eilber Fritz C.、Hoffman Robert M.
  • Journal Title: Cell Cycle Volume: 16 Issue: 12 Pages: 1164-1170
  • DOI: 10.1080/15384101.2017.1317417
  • Peer Reviewed
• [Journal Article] Antimetastatic Efficacy of the Combination of Caffeine and Valproic Acid on an Orthotopic Human Osteosarcoma Cell Line Model in Nude Mice (2017)
  • Author(s): Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Hoffman RM
  • Journal Title: Anticancer Res Volume: 37 Pages: 1005-1011
  • Peer Reviewed
• [Journal Article] Phase 1/2 study of immunotherapy with dendritic cells pulsed with autologous tumor lysate in patients with refractory bone and soft tissue sarcoma. (2017)
  • Author(s): Miwa S, Nishida H, Tanzawa Y, Takeuchi A, Hayashi K, Yamamoto N, Mizukoshi E, Nakamoto Y, Kaneko S, Tsuchiya H.
  • Journal Title: Cancer Volume: 123 Issue: 9 Pages: 1576-84
  • DOI: 10.1002/cncr.30606
  • Peer Reviewed
• [Journal Article] GFP labeling kinetics of triple-negative human breast cancer by a killer-reporter adenovirus in 3D Gelfoam? histoculture (2017)
  • Author(s): Yano Shuya、Takehara Kiyoto、Miwa Shinji、Kishimoto Hiroyuki、Tazawa Hiroshi、Urata Yasuo、Kagawa Shunsuke、Bouvet Michael、Fujiwara Toshiyoshi、Hoffman Robert M.
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 53 Issue: 6 Pages: 479-482
  • DOI: 10.1007/s11626-017-0133-6
  • Peer Reviewed
• [Journal Article] Pathogenesis of Osteosclerotic Change Following Treatment with an Antibody Against RANKL for Giant Cell Tumour of the Bone (2017)
  • Author(s): Rosario M, Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Higuchi T, Abe K, Taniguchi Y, Aiba H, Tanzawa Y, Murakami H, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 37 Pages: 749-754
  • Peer Reviewed
• [Journal Article] High Efficacy of Pazopanib on an Undifferentiated Spindle-Cell Sarcoma Resistant to First-Line Therapy Is Identified With a Patient-Derived Orthotopic Xenograft (PDOX) Nude Mouse Model (2017)
  • Author(s): Igarashi Kentaro、Kawaguchi Kei、Murakami Takashi、Kiyuna Tasuku、Miyake Kentaro、Singh Arun S.、Nelson Scott D.、Dry Sarah M.、Li Yunfeng、Yamamoto Norio、Hayashi Katsuhiro、Kimura Hiroaki、Miwa Shinji、Tsuchiya Hiroyuki、Eilber Fritz C.、Hoffman Robert M.
  • Journal Title: J Cell Biochem Volume: 118 Issue: 9 Pages: 2739-2743
  • DOI: 10.1002/jcb.25923
  • Peer Reviewed
• [Journal Article] Practical use of imaging technique for management of bone and soft tissue tumors (2017)
  • Author(s): Miwa Shinji、Otsuka Takanobu
  • Journal Title: J Orthop Sci Volume: 22 Issue: 3 Pages: 391-400
  • DOI: 10.1016/j.jos.2017.01.006
  • NAID: 40021366082
  • Peer Reviewed / Open Access
• [Journal Article] Do Mesenchymal Stem Cells Derived From Atypical Lipomatous Tumors Have Greater Differentiation Potency Than Cells From Normal Adipose Tissues? (2017)
  • Author(s): Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Asai K, Otsuka T, Tsuchiya H.
  • Journal Title: Clin Orthop Relat Res Volume: 印刷中 Issue: 6 Pages: 1693-1701
  • DOI: 10.1007/s11999-017-5259-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model (2017)
  • Author(s): Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh A, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM
  • Journal Title: Cell Cycle Volume: 16 Issue: 1 Pages: 91-94
  • DOI: 10.1080/15384101.2016.1252885
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Surgical management of proximal fibular tumors: A report of 12 cases (2016)
  • Author(s): Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Okamoto H, Otsuka T, Tsuchiya H
  • Journal Title: J Bone Oncol Volume: 5 Issue: 4 Pages: 163-166
  • DOI: 10.1016/j.jbo.2016.06.001
  • NAID: 120005982984
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Outcome of Reconstruction Using Frozen Autograft for a Humeral Bone Tumor (2016)
  • Author(s): Kimura H, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Takeuchi A, Miwa S, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 36 Pages: 6631-6635
  • Peer Reviewed
• [Journal Article] Efficacy of glycogen synthase kinase-3β targeting against osteosarcoma via activation of β-catenin. (2016)
  • Author(s): Shimozaki S, Minamoto T, et al.
  • Journal Title: Oncotarget Volume: 7 Issue: 47 Pages: 77038-51
  • DOI: 10.18632/oncotarget.12781
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Non-toxic Efficacy of the Combination of Caffeine and Valproic Acid on Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models (2016)
  • Author(s): Igarashi K, Yamamoto N, Hayashi K, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 36 Pages: 4477-82
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Efficacy of the Combination of a PARP Inhibitor and UVC on Cancer Cells as Imaged by Focus Formation by the DNA Repair-related Protein 53BP1 Linked to Green Fluorescent Protein (2016)
  • Author(s): Tome Y, Uehara F, Miwa S, Yano S, Mii S, Efimova EV, Bouvet M, Kimura H, Tsuchiya H, Kanaya F, Hoffman RM
  • Journal Title: Anticancer Res Volume: 36 Pages: 3821-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Use of αv Integrin Linked to Green Fluorescent Protein in Osteosarcoma Cells and Confocal Microscopy to Image Molecular Dynamics During Lung Metastasis in Nude Mice (2016)
  • Author(s): Tome Y, Yano S, Sugimoto N, Mii S, Uehara F, Miwa S, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM
  • Journal Title: Anticancer Res Volume: 36 Pages: 3811-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clinical relevance of peroxisome proliferator-activated receptor-gamma expression in myxoid liposarcoma (2016)
  • Author(s): Takeuchi A, Yamamoto N, Shirai T, Hayashi K, Miwa S, Munesue S, Yamamoto Y, Tsuchiya H
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 442-442
  • DOI: 10.1186/s12885-016-2524-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Balancing Prolonged Survival with QoL Using Low-dose Pazopanib Maintenance: A Comparison with the PALETTE Study (2016)
  • Author(s): Abe K, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Inatani H, Higuchi T, Taniguchi Y, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 36 Pages: 2893-7
  • Peer Reviewed
• [Journal Article] The outcomes of reconstruction using frozen autograft combined with iodine-coated implants for malignant bone tumors: compared with non-coated implants (2016)
  • Author(s): Shirai T, Tsuchiya H, Terauchi R, Tsuchida S, Mizoshiri N, Igarashi K, Miwa S, Takeuchi A, Kimura H, Hayashi K, Yamamoto N, Kubo T
  • Journal Title: Jpn J Clin Oncol Volume: 46 Issue: 8 Pages: 735-40
  • DOI: 10.1093/jjco/hyw065
  • NAID: 120005899104
  • Peer Reviewed
• [Journal Article] Tenosynovial giant cell tumors in unusual locations detected by positron emission tomography imaging confused with malignant tumors: report of two cases (2016)
  • Author(s): Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Takahira M, Fukui K, Oikawa T, Tsuchiya H
  • Journal Title: BMC Musculoskelet Disord Volume: 17 Issue: 1 Pages: 180-180
  • DOI: 10.1186/s12891-016-1050-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Real-time in vivo confocal fluorescence imaging of prostate cancer bone-marrow micrometastasis development at the cellular level in nude mice (2016)
  • Author(s): Miwa S, Toneri M, Igarashi K, Yano S, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 117 Issue: 11 Pages: 2533-2537
  • DOI: 10.1002/jcb.25545
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescence-guided surgery of human prostate cancer experimental bone metastasis in nude mice using anti-CEA DyLight 650 for tumor illumination. (2016)
  • Author(s): Miwa S, De Magalhaes N, Toneri M, Zhang Y, Cao W, Bouvet M, Tsuchiya H, Hoffman RM.
  • Journal Title: Journal of Orthopedic Research Volume: 34 Issue: 4 Pages: 559-65
  • DOI: 10.1002/jor.22973
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma (2017)
  • Author(s): Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Higuchi T, Abe K, Taniguchi Y, Tsuchiya H
  • Organizer: 19th International Society of Limb Salvage General Meeting
  • Int'l Joint Research
• [Presentation] Deep infection after bone tumor surgery: risk factors and new preventive technology (2017)
  • Author(s): Miwa S, Shirai T, Yamamoto N, Hayashi K, Takeuchi A, Tada K, Kajino Y, Higuchi T, Abe K, Taniguchi Y, Tsuchiya H
  • Organizer: 19th International Society of Limb Salvage General Meeting
  • Int'l Joint Research
• [Presentation] Deep Infection After Bone Tumor Surgery: Risk Factors And New Preventive Technology (2017)
  • Author(s): Miwa S, Shirai T, Yamamoto N, Hayashi K, Takeuchi A, Tada K, Kajino Y, Higuchi T, Abe K, Taniguchi Y, Tsuchiya H
  • Organizer: 18th EFORT Annual Congress
  • Int'l Joint Research
• [Presentation] 難治性肉腫に対する樹状細胞を用いた免疫療法についての検討 (2017)
  • Author(s): 三輪真嗣，土屋弘行
  • Organizer: 第26回がん転移学会
• [Presentation] 骨腫瘍における術後感染の危険因子とヨード担持インプラントの有効性 (2017)
  • Author(s): 三輪真嗣，白井寿治，山本憲男，林克洋，武内章彦，土屋弘行
  • Organizer: 第50回日本整形外科学会骨・軟部腫瘍学術集会
• [Presentation] 難治性肉腫に対する樹状細胞を用いた臨床試験 (2017)
  • Author(s): 三輪真嗣，西田英司，丹沢義一，武内章彦，山本憲男，土屋弘行
  • Organizer: 第50回日本整形外科学会骨・軟部腫瘍学術集会
• [Presentation] 骨転移病変に対する液体窒素処理骨を用いた再建術の検討 (2016)
  • Author(s): 三輪真嗣，土屋弘行
  • Organizer: 第25回がん転移学会
  • Place of Presentation: 米子コンベンションセンター（鳥取県・米子市）
  • Year and Date: 2016-07-21
• [Presentation] 骨軟部病変の良悪性鑑別における18F-FDG-PETの有用性の検討 (2016)
  • Author(s): 三輪真嗣，山本憲男，林克洋，武内章彦，稲谷弘幸，樋口貴史，阿部健作，谷口悠太，土屋弘行
  • Organizer: 第49回日本整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: 東京ドームホテル（東京都文京区）
  • Year and Date: 2016-07-14
• [Presentation] Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model (2016)
  • Author(s): Miwa S, Uehara F, Kimura H, Hayashi K, Yamamoto N, Hoffman RM, Tsuchiya H.
  • Organizer: 17th EFORT Annual Congress
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2016-06-01
  • Int'l Joint Research