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Transcriptional regulation of the fibrillar collagen genes, type XI and XXVII collagens, expressed in chondrocytes

Research Project

Project/Area Number 16K20064
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionOita University

Principal Investigator

Mariko Hida  大分大学, 医学部, 客員研究員 (10737224)

Research Collaborator Matsuo Noritaka  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsコラーゲン / 軟骨 / 転写 / 骨
Outline of Final Research Achievements

In this study, we investigated the proximal promoter of Col11a1 and Col27a1 gene in chondrocytes.In Col11a1 gene, cell transfection experiments exhibited the suppression of the promoter activity without NF-Y binding sequence. Luciferase assays exhibited that GC rich sequence is a critical elements. Overexpression of Sp1 was significantly increased, and knockdown of Sp1 was suppressed the expression of endogenous transcript. Taken together, the transcription factor NF-Y and Sp1 upregulates the proximal promoter activity of Col11a1 gene in chondrocytes.In Col27a1 gene, oligo-Capping Race analysis revealed that mouse Col27a1 gene has two alternative promoters in chondrocytes. Transient transfection experiments indicated that the proximal promoter activity was from -586 to -445 in the downstream promoter and was from -310 to +1 in the upstream promoter. However, the mechanism of col27a1 gene regulation in chondrocytes has not been understood in this study.

Academic Significance and Societal Importance of the Research Achievements

申請者らは、活性領域の同定が困難とされていた組織特異的シスエレメントの網羅的スクリーニング法を開発し、これまで報告のない2つの線維性コラーゲン遺伝子の軟骨特異的エンハンサー領域を発見する事ができた。本研究の進展により、軟骨における分子発現機構や軟骨分化メカニズムの解明に貢献するだけでなく、軟骨形成不全症の診断や治療に新知見を与える事ができ、再生医療への応用も期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] GABA promotes elastin synthesis and elastin fiber formation in normal human dermal fibroblasts (HDFs)2017

    • Author(s)
      Uehara E, Hokazono H, Hida M, Sasaki T, Yoshioka H, Matsuo N
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 81 Pages: 1198-1205

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 1)Sp1 upregulated the proximal promoter activity of the mouse collagen a1(XI) gene (Col11a1) in chondrocytes.2016

    • Author(s)
      Watanabe K, Hida M, Sasaki T, Yana H, Kawano K, Yoshioka H, Matsuo N.
    • Journal Title

      In Vitro Cell Dev Biol Anim

      Volume: 52 Issue: 2 Pages: 235-42

    • DOI

      10.1007/s11626-015-9959-y

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 1)XXVII型コラーゲンα1鎖遺伝子(Col27a1)の転写調節機構の解析2017

    • Author(s)
      樋田真理子、佐藤はるな、佐々木隆子、吉岡秀克、松尾哲孝
    • Organizer
      日本生化学会、九州支部例会
    • Related Report
      2017 Research-status Report
  • [Presentation] 転写因子Sp1は軟骨におけるCol11a1の基本プロモーター活性を制御する2016

    • Author(s)
      渡辺啓次郎、樋田真理子、佐藤はるな、佐々木隆子、吉岡秀克、松尾哲孝
    • Organizer
      第48回日本結合組織学会
    • Place of Presentation
      長崎大学(長崎県、長崎市)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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