Smoking cessation affects human platelet activation

• Project/Area Number: 16K20089
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: Gifu University
• Principal Investigator: Kito Yuko 岐阜大学, 大学院医学系研究科, 特任助教 (30770755)
• Research Collaborator: Kozawa Osamu ; Iida Hiroki ; Iida Mami ; Onuma Takashi ; Tsujimoto Masanori
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 血小板 / collagen / ADP / 禁煙 / 抗血小板薬 / 血小板機能 / 心・脳血管疾患 / ELISA法 / Western blot法

Outline of Final Research Achievements

Our results strongly suggest that smoking cessation causes temporary hyper-activation of human platelets in the short term. We may be able to reduce the incidence of complications due to hyper-reactivity of human platelets by considering the smoking abstinence period.

Academic Significance and Societal Importance of the Research Achievements

禁煙開始から12週まで4週ごとに血液検査を実施し、禁煙実施患者におけるcollagen、ADPによって引き起こされる血小板の凝集について評価した。また、凝集能の変化、凝集反応が起こる際に分泌されるPDGF-ABやsCD40Lの変化を計測した。禁煙することは血小板の凝集に対して一時的に過凝固を引き起こす可能性がある。周術期において禁煙の時期を考えることは禁煙によって惹起される様々な合併症を減らすことができる可能性がある。

Research Products

• [Journal Article] Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor (2017)
  • Author(s): Onuma Takashi、Tanabe Kumiko、Kito Yuko、Tsujimoto Masanori、Uematsu Kodai、Enomoto Yukiko、Matsushima-Nishiwaki Rie、Doi Tomoaki、Nagase Kiyoshi、Akamatsu Shigeru、Tokuda Haruhiko、Ogura Shinji、Iwama Toru、Kozawa Osamu、Iida Hiroki
  • Journal Title: Thrombosis Research Volume: 156 Pages: 91-100
  • DOI: 10.1016/j.thromres.2017.06.001
  • Peer Reviewed
• [Journal Article] Factor Xa inhibitor suppresses the release of phosphorylated HSP27 from collagen-stimulated human platelets: inhibition of HSP27 phosphorylation via p44/p42 MAP kinase. (2016)
  • Author(s): Tsujimoto M, Kuroyanagi G, Matsushima-Nishiwaki R, Kito Y, Enomoto Y, Iida H, Ogura S, Otsuka T, Tokuda H, Kozawa O, Iwama T
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0149077-e0149077
  • DOI: 10.1371/journal.pone.0149077
  • Peer Reviewed / Open Access
• [Journal Article] . AICAR reduces collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: suppression of HSP27 phosphorylation via p44/p42 MAP kinase (2016)
  • Author(s): 6.Tsujimoto M, Tokuda H, Kuroyanagi G, Yamamoto N, Kainuma S, Matsushima-Nishiwaki R, Onuma T, Iida Y, Kojima A, Sawada S, Doi T, Enomoto Y, Tanabe K, Akamatsu S, Iida H, Ogura S, Otsuka T, Kozawa O, Iwama T
  • Journal Title: . Exp Ther Med. Volume: 12 Pages: 1107-1112
  • Peer Reviewed / Open Access