Elucidation of extracellular matrix molecular pathway regulated by metastasis-suppressing microRNAs in prostate cancer.

• Project/Area Number: 16K20125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Chiba University
• Principal Investigator: KATO MAYUKO 千葉大学, 医学部附属病院, 助教 (80733857)
• Co-Investigator(Renkei-kenkyūsha): ICHIKAWA TOMOHIKO 千葉大学, 大学院医学研究院, 教授 (20241953) ; SEKI NAOHIKO 千葉大学, 大学院医学研究院, 准教授 (50345013) ; KOJIMA SATOKO 帝京大学, 医学部, 准教授 (10345019) ; OKATO ATSUSHI 千葉大学, 医学部附属病院, 医員 (90756719) ; ARAI TAKAYUKI 千葉大学, 医学部附属病院, 医員 (40793055) ; KOSHIZUKA KEIICHI 千葉大学, 医学部附属病院, 医員 (00791609) ; YAMADA YASUTAKA 千葉大学, 医学部附属病院, 医員 (30814595) ; SUGAWARA SYO 千葉大学, 医学部附属病院, 医員 (70815290)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マイクロRNA / 前立腺癌 / 細胞外マトリックス / 去勢抵抗性前立腺癌 / 癌抑制遺伝子 / 癌遺伝子

Outline of Final Research Achievements

microRNA(miRNA) expression profile in castration-resistant prostate cancer(CRPC) revealed that miR-205-5p was reduced in CRPC tissues and that it acted as an antitumor miRNA. We focused on HMGB3 as a putative target of miR-205-5p regulation. HMGB3 was directly regulated by miR-205-5p in PCa cells and HMGB3 was overexpressed in CRPC clinical specimens.

Research Products

• [Journal Article] Regulation of HMGB3 by antitumor miR-205-5p inhibits cancer cell aggressiveness and is involved in prostate cancer pathogenesis (2017)
  • Author(s): Yamada Yasutaka、Nishikawa Rika、Kato Mayuko、Okato Atsushi、Arai Takayuki、Kojima Satoko、Yamazaki Kazuto、Naya Yukio、Ichikawa Tomohiko、Seki Naohiko
  • Journal Title: J Hum Genet Volume: 63 Issue: 2 Pages: 195-205
  • DOI: 10.1038/s10038-017-0371-1
  • Peer Reviewed / Open Access
• [Journal Article] Impact of novel miR-145-3p regulatory networks on survival in patients with castration-resistant prostate cancer (2017)
  • Author(s): Goto Yusuke、Kurozumi Akira、Arai Takayuki、Nohata Nijiro、Kojima Satoko、Okato Atsushi、Kato Mayuko、Yamazaki Kazuto、Ishida Yasuo、Naya Yukio、Ichikawa Tomohiko、Seki Naohiko
  • Journal Title: British Journal of Cancer Volume: 117 Issue: 3 Pages: 409-420
  • DOI: 10.1038/bjc.2017.191
  • Peer Reviewed
• [Journal Article] Dual strands of pre-miR-150 (miR-150-5p and miR-150-3p) act as antitumor miRNAs targeting SPOCK1 in na?ve and castration-resistant prostate cancer (2017)
  • Author(s): Okato Atsushi、Arai Takayuki、Kojima Satoko、Koshizuka Keiichi、Osako Yusaku、Idichi Tetsuya、Kurozumi Akira、Goto Yusuke、Kato Mayuko、Naya Yukio、Ichikawa Tomohiko、Seki Naohiko
  • Journal Title: International Journal of Oncology Volume: 51 Issue: 1 Pages: 245-256
  • DOI: 10.3892/ijo.2017.4008
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of metastasis-promoting LOXL2 gene expression by antitumor microRNAs in prostate cancer. (2017)
  • Author(s): Kato M, Kurozumi A, Goto Y, Matsushita R, Okato A, Nishikawa R, Fukumoto I, Koshizuka K, Ichikawa T, Seki N.
  • Journal Title: Journal of Human genetics Volume: 62 Pages: 123-132
  • NAID: 40021036352
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer (2016)
  • Author(s): Okato A, Goto Y, Kurozumi A, Kato M, Kojima S, Matsushita R, Yonemori M, Miyamoto K, Ichikawa T, Seki N.
  • Journal Title: International journal of oncology Volume: 49 Pages: 111-22
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of E3 ubiquitin ligase-1 (WWP1) by microRNA-452 inhibits cancer cell migration and invasion in prostate cancer. (2016)
  • Author(s): Goto Y, Kojima S, Kurozumi A, Kato M, Okato A, Matsushita R, Ichikawa T, Seki N.
  • Journal Title: British journal of cancer Volume: 114 Pages: 1135-44
  • Peer Reviewed / Open Access
• [Presentation] Tumor-suppressive microRNAs (miR-26a/b, miR-29a/b/c and miR-218) concertedly regulate metastasis-promoting LOXL2 in prostate cancer (2017)
  • Author(s): Mayuko Kato, Akira Kurozumi, Yusuke Goto, Ryosuke Matsushita, Ichiro Fukumoto, Atsushi Okato, Rika Nishikawa, Shinichi Sakamoto, Tomohiko Ichikawa, Naohiko Seki
  • Organizer: American Association for Cancer Research Annual meeting 2016
  • Place of Presentation: Ernest N. Morial Convention Center New Orleans, Louisiana, USA
  • Year and Date: 2017-04-18
  • Int'l Joint Research
• [Presentation] Dual-strand tumor-suppressor microRNA-145 (miR-145-5p and miR-145-3p) are involved in castration-resistant prostate cancer pathogenesis (2017)
  • Author(s): Mayuko Kato, Akira Kurozumi, Yusuke Goto, Nijiro Nohata, Takayuki Arai, Atsushi Okato, Keiichi Koshizuka, Satoko Kojima, Tomohiko Ichikawa, Naohiko Seki.
  • Organizer: American Association for Cancer Research
  • Int'l Joint Research