| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Whole blood was collected from 12 RCC patients and 12 healthy volunteers as controls. Whole blood was collected directly into a PAXgene RNA tube to instantly stabilize RNA, and we performed gene expression profiling using microarrays. We confirmed the microarray results using qRT-PCR, then selected candidate genes for diagnosing RCC. We then verified the predictive ability using a verification cohort (n=180).
We identified 15 genes differentially expressed in RCC patients versus controls. We focused on CXCL7, which was upregulated in RCC patients. Receiver Operatorating Characteristic curve analysis showed that the expression level of CXCL7 in peripheral blood cells was useful to diagnose RCC patients in the verification cohort (AUC=0.722).
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