Functional analysis of bone marrow-derived mesenchymal stem cells in renal cell carcinoma microenvironment

• Project/Area Number: 16K20142
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Hiroshima University
• Principal Investigator: Kitano Hiroyuki 広島大学, 病院(医), 助教 (60721933)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 骨髄由来間葉系幹細胞 / 腎細胞癌 / 癌微小環境 / 癌間質 / 癌

Outline of Final Research Achievements

Bone marrow　derived mesenchymal stem cells were labeled with PKH Red Fluoresce nt. Orthotopically transplanted model mice were prepared using a renal cell car cinoma cell line of a luciferase stable expression strain, and MSCs labeled fro m the tail vein were intravenously administered. Currently,　differentiated cel ls of MSC are being identified by immunostaining using a cofocal microscope. Ca ki and MSC were cocultured under the observation of IncuCyte Zoom. Caki did not show an increase in cell proliferation ability when cultured under the conditio n medium of MSC, but was elevated by direct contact with MSC. Subsequently, cha nges in migratory ability are under consideration.