| Project/Area Number |
16K20164
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 再生医療 / 尿失禁 / 骨格筋幹細胞 / 泌尿器 / 前立腺全摘除 / パラクライン効果 / Tissue Engineering / 尿道括約筋 / 骨格筋由来幹細胞 / 体性幹細胞 / 移植・再生医療 |
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| Outline of Final Research Achievements |
Postoperative damage of the urethral sphincter is a major complication of prostatectomy and causes urinary incontinence. Human skeletal muscle-derived stem cells (Sk-SCs) were applied to this damage. Sk-SCs were extracted from the human rectus abdominis, sorted as CD34+/45-(Sk-34) and CD29+/34-/45-(Sk-DN/29+). Urethral damage was induced by removing the muscle layer in nude rats. A mixture of Sk-34 and Sk-DN/29+ cells was applied on the damaged portion. Urethral pressure was evaluated to assess functional recovery. Cell engraftments and differentiations were detected. Expression of angiogenic cytokines was also analyzed. The transplantation group showed a higher functional recovery. Human Sk-SCs differentiated into skeletal muscle, Schwann cells, perineurium, and vascular cells. Active paracrine angiogenic cytokines were also detected with enhanced vascular formation in vivo. The Sk-SCs is useful for the reconstitution of postoperative damage of the urethral sphincter.
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