| Project/Area Number |
16K20172
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 卵巣癌 / TERTプロモーター変異 / TERTプロモーター / 癌 |
|---|
| Outline of Final Research Achievements |
Cell proliferation by addition of vitamin A and vitamin D to ovarian clear cell adenocarcinoma 4 strains showed cytostatic effect in OVISE, OVTOKO and TOV - 21G cell lines. It was thought that vitamin A and vitamin D receptor were bound and transcription of telomerase was suppressed, and cell proliferation inhibitory effect was seen. On the other hand, cell proliferation inhibitory effect was not observed in ES-2 cell line. Since ES-2 is a cell line with a mutation in the TERT promoter [-129 to -154 bp] region, it was thought that the inhibition of vitamin A, D was canceled and transcription of telomerase was enhanced. In addition, chromatin immunoprecipitation was performed using the TOV-21 G cell line, which is without TERT promoter mutation, and it was confirmed that the RARα antibody binds to the TERT promoter region. The [-129 to -154 bp] region was shown to have a binding site for the vitamin A receptor.
|
