Pathological analysis of immortalized endometriotic cells by introducing sex hormone receptor genes

• Project/Area Number: 16K20180
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Kanazawa University
• Principal Investigator: BONO YUKIKO 金沢大学, 医学系, 協力研究員 (80565416)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 子宮内膜症 / 不死化細胞 / 性ステロイドホルモン / 不死化子宮内膜間質細胞 / ステロイドホルモン

Outline of Final Research Achievements

Purified stromal cells isolated from ovarian endometriomas were successfully immortalised by combinatorial transfection of human cdk4 and human telomerase reverse transcriptase (hTERT) genes or hTERT genes. These cells expressed progesterone receptor B and showed significant growth inhibition by progestin.
We generated immortalised epithelial and stromal cells from ovarian endometrioma that retained sex steroid responsiveness. These cells are invaluable tools not only for the consistent in vitro work but also for the study of molecular pathogenesis of endometriosis.