The detection of cholesteatoma residue by using immunofluorescent method during surgery
| Project/Area Number |
16K20251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Ehime University |
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Principal Investigator |
Takagi Daiki 愛媛大学, 医学系研究科, 助教 (10448376)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 真珠腫性中耳炎 / 術中診断 / galectin-7 / 術中蛍光診断 / 蛍光顕微鏡 / 外科 |
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| Outline of Final Research Achievements |
The purpose of this study is to develop how to visualize the residual cholesteatomas at the time of operation by using immunofluorescent method with laser microscope. We tried antibody purification to develop a fluorescent reagent, but it is difficult from the aspect of cost required for development. Therefore, as a staining method without using antibodies, we studied changing the excitation wavelength of the fluorescence microscope.
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Report
(3 results)
Research Products
(1 results)
