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Usefulness of disease-specific microRNA for respiratory syncytial virus induced lower respiratory tract inflammation

Research Project

Project/Area Number 16K20266
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionSapporo Medical University

Principal Investigator

Yamamoto Keisuke  札幌医科大学, 医学部, 助教 (50738515)

Research Collaborator HIMI Tetsuo  札幌医科大学, 医学部, 名誉教授 (90181114)
YOKOTA Shin-ichi  札幌医科大学, 医学部, 教授 (10325863)
Takano Kenichi  札幌医科大学, 医学部, 准教授 (70404689)
KUROSE Makoto  札幌医科大学, 医学部, 講師 (60404696)
KAMEKURA Ryuta  札幌医科大学, 医学部, 講師 (70404697)
OHKUNI Tsuyoshi  札幌医科大学, 医学部, 助教 (40464490)
OGASAWARA Noriko  札幌医科大学, 医学部, 助教 (00438061)
YAMAMOTO Soh  札幌医科大学, 医学部, 助教 (10588479)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsRSウイルス / III型インターフェロン / クラリスロマイシン / IRF-3 / 鼻汁 / microRNA / housekeeping gene / プラーク法 / reference microRNA / ハウスキーピング / RS ウイルス / 自然免疫 / RSウィルス
Outline of Final Research Achievements

(1)Clarithromycin (CAM) as a treatment of RSV infection and (2)disease-specific microRNA as an index of disease evaluation were examined.CAM treatment led to a significant reduction in RSV-mediated IL-8, CCL5, IFN-βand -λ production.Furthermore,IFN-β promoter activity (activated by poly I:C and RSV infection) was significantly reduced after treatment with CAM.CAM also inhibited IRF-3 dimerization and subsequent translocation to the nucleus.

RSV infection-specific secreted miRNAs were identified. Housekeeping gene of nasal secreted miRNA was identified.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results) Presentation (5 results)

  • [Journal Article] Evaluation of consistency in quantification of gene copy number by real-time reverse transcription quantitative polymerase chain reaction and virus titer by plaque-forming assay for human respiratory syncytial virus.2018

    • Author(s)
      Yamamoto K, Ogasawara N, Yamamoto S, Takano K, Shiraishi T, Sato T, Tsutsumi H, Himi T, Yokota SI.
    • Journal Title

      Microbiol Immunol.

      Volume: 62 Issue: 2 Pages: 90-98

    • DOI

      10.1111/1348-0421.12563

    • NAID

      40021481337

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial proteins NIP-SNAP-1 and -2 are a target for the immunomodulatory activity of clarithromycin, which involves NF-κB-mediated cytokine production.2017

    • Author(s)
      Yamamoto S, Ogasawara N, Yamamoto K, Uemura C, Takaya Y, Shiraishi T, Sato T, Hashimoto S, Tsutsumi H, Takano K, Himi T, Yokota SI.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 483 Issue: 3 Pages: 911-916

    • DOI

      10.1016/j.bbrc.2016.12.100

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Clarithromycin prevents human respiratory syncytial virus-induced airway epithelial responses by modulating activation of interferon regulatory factor-3.2016

    • Author(s)
      Yamamoto K, Yamamoto S, Ogasawara N, Takano K, Shiraishi T, Sato T, Miyata R, Kakuki T, Kamekura R, Kojima T, Tsutsumi H, Himi T, Yokota S.
    • Journal Title

      Pharmacol Res

      Volume: 111 Pages: 804-814

    • DOI

      10.1016/j.phrs.2016.07.033

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mumps Virus Induces Protein-Kinase-R-Dependent Stress Granules, Partly Suppressing Type III Interferon Production.2016

    • Author(s)
      Hashimoto S, Yamamoto S, Ogasawara N, Sato T, Yamamoto K, Katoh H, Kubota T, Shiraishi T, Kojima T, Himi T, Tsutsumi H, Yokota S
    • Journal Title

      PLoS One.

      Volume: Aug 4;3: Issue: 8 Pages: 16023-16023

    • DOI

      10.1371/journal.pone.0161793

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] クラリスロマイシンは気道上皮でRSVによって誘導されるインターフェロンをIRF-3を介して調整する2017

    • Author(s)
      山本圭佑,小笠原徳子,高野賢一,宮田遼,角木拓也,亀倉隆太,氷見徹夫
    • Organizer
      第118回日本耳鼻咽喉科学会通常総会・学術講演会
    • Place of Presentation
      広島国際会議場(広島県広島市)
    • Year and Date
      2017-05-17
    • Related Report
      2016 Research-status Report
  • [Presentation] クラリスロマイシンは気道上皮細胞で RS ウイルスによって誘導されるインターフェロンの産生を IRF-3 を介して調整する2017

    • Author(s)
      山本圭佑, 小笠原徳子, 高野賢一, 氷見徹夫
    • Organizer
      第35回日本耳鼻咽喉科免疫アレルギー学会
    • Place of Presentation
      旭川グランドホテル(北海道旭川市)
    • Year and Date
      2017-04-13
    • Related Report
      2016 Research-status Report
  • [Presentation] ウイルス感染症での鼻粘膜上皮における細気管支炎・喘鳴の発症予測因子としての鼻汁 microRNA の可能性2017

    • Author(s)
      山本圭佑,小笠原徳子,大國毅,高野賢一,堤裕幸,氷見徹夫
    • Organizer
      第 56 回 日本鼻科学会 総会・学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クラリスロマイシンは気道上皮でRSVによって誘導されるインターフェロンをIRF-3を介して調整する2017

    • Author(s)
      山本圭佑,小笠原徳子,高野賢一,宮田遼,角木拓也,亀倉隆太,氷見徹夫
    • Organizer
      第 118 回 日本耳鼻咽喉科学会通常総会・学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Clarithromycin prevents human respiratory syncytial virus-induced airway epithelial responses by modulating activation of interferon regulatory factor-32016

    • Author(s)
      Keisuke Yamamoto, Hiroyuki Tsutsumi, Tetsuo Himi, Shin-ichi Yokota
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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