| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The human nasal mucosa forms a continuous barrier via tight junctions. The paracellular pathway regulated by tight junctions is important in intranasal administration across the nasal mucosa. Clostridium perfringens enterotoxin (C-CPE) mutants C-CPE194 and C-CPEm19 disrupted the tight junctional barrier without a cytotoxic effect and regulated the permeability of insulin across human nasal epithelial cells. Also, miRNA-146a induced by TLR ligand poly(I:C) enhanced the epithelial barrier and acted as a negative regulator of inflammatory responses in human nasal epithelial cells. This study suggests that C-CPE194, C-CPEm19 and miRNA-146a may play a key role in developing a safety and adjustable novel drug delivery system across the nasal mucosa.
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