Construction of a novel drug delivery system across the human nasal mucosa

• Project/Area Number: 16K20267
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Sapporo Medical University
• Principal Investigator: YAJIMA RYOTO 札幌医科大学, 医学部, 研究員 (90722455)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: タイト結合分子バインダー / C-CPE / microRNA / ヒト鼻粘膜上皮細胞 / インスリン / 細胞・組織 / トランスレーショナルリサーチ

Outline of Final Research Achievements

The human nasal mucosa forms a continuous barrier via tight junctions. The paracellular pathway regulated by tight junctions is important in intranasal administration across the nasal mucosa. Clostridium perfringens enterotoxin (C-CPE) mutants C-CPE194 and C-CPEm19 disrupted the tight junctional barrier without a cytotoxic effect and regulated the permeability of insulin across human nasal epithelial cells. Also, miRNA-146a induced by TLR ligand poly(I:C) enhanced the epithelial barrier and acted as a negative regulator of inflammatory responses in human nasal epithelial cells. This study suggests that C-CPE194, C-CPEm19 and miRNA-146a may play a key role in developing a safety and adjustable novel drug delivery system across the nasal mucosa.

Research Products

• [Journal Article] A Novel Drug Delivery System for the Human Nasal Epithelium. (2016)
  • Author(s): Takano K, Kojima T, Keira T, Miyata R, Nomura K, Kakuki T, Kaneko Y, Yajima R, Kakiuchi A, Himi T.
  • Journal Title: Adv Otorhinolaryngol. Volume: 77 Pages: 67-74
  • DOI: 10.1159/000441877
  • ISBN: 9783318056501, 9783318056518
  • Peer Reviewed