• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of novel peptides for protecting retinal ganglion cells.

Research Project

Project/Area Number 16K20298
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionIwate University

Principal Investigator

Ozaki Taku  岩手大学, 理工学部, 准教授 (70621069)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywordsペプチド / 点眼薬 / 緑内障 / 網膜神経節細胞 / ミトコンドリア / カルパイン / モデルマウス / 神経保護 / 点眼 / 緑内障モデルマウス / NMDA硝子体注入マウス / 視神経軸索障害マウス / NMDA誘導性網膜傷害 / C57BL/6マウス / アマクリン細胞 / ペプチド点眼 / TUNEL試験 / フラットマウントイメージング / 保護効果 / 器官培養 / 網膜 / NMDA / RGC-5細胞 / ミトコンドリアカルパイン / 阻害ペプチド / Tat-μCL / 細胞死 / アポトーシス / ネクローシス
Outline of Final Research Achievements

The purpose of this study was to develop a novel eye drops for glaucoma. We have recently succeeded in developing a peptide that specifically inhibits mitochondrial calpain-1, which is related to cell death in the retina. The peptide was delivered to the retina and optic disc by eye drops, and a protective effect on photoreceptor cells was observed in an animal model of retinitis pigmentosa. A protective effect was also observed against ganglion cell death in the ocular hypertension / ischemia. In the present study, the efficacy of the peptide was confirmed by comprehensively evaluating the effect of the peptide on ganglion cell death using cultured cells, organ culture retina, and glaucoma model mouse.

Academic Significance and Societal Importance of the Research Achievements

現在の緑内障治療は眼圧下降を目的として行われているが、眼圧が正常または眼圧を充分に下降させてもなお視野障害が進行する症例が本邦の約7割を占めるため、神経節細胞の保護を目的とした治療薬の開発が強く望まれている。申請者らは以前、緑内障性神経節細胞死においてミトコンドリアカルパインとAIFが関与していることを世界に先駆けて証明した。さらに、本研究により、新規緑内障治療薬の候補薬剤を新たに生み出したことで、非臨床試験へと展開できる可能性が見出された。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Presence of calpain-5 in mitochondria2018

    • Author(s)
      Takeshi Iwamoto, Eri Ishiyama, Kinji Ishida, Tetsuro Yamashita, Hiroshi Tomita, Taku Ozaki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 504 Issue: 2 Pages: 454-459

    • DOI

      10.1016/j.bbrc.2018.08.144

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Kinetic profiles of photocurrents in cells expressing two types of channelrhodopsin genes.2018

    • Author(s)
      Watanabe Y, Sugano E, Tabata K, Ozaki T, Saito T, Tamai M, Tomita H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 12;496(3) Issue: 3 Pages: 814-819

    • DOI

      10.1016/j.bbrc.2018.01.149

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visual Responses of Photoreceptor-Degenerated Rats Expressing Two Different Types of Channelrhodopsin Genes2017

    • Author(s)
      Masatoshi Sato, Eriko Sugano, Kitako Tabata, Kei Sannohe, Yoshito Watanabe, Taku Ozaki, Makoto Tamai, Hiroshi Tomita
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 41210-41210

    • DOI

      10.1038/srep41210

    • NAID

      120007176801

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The physiological accumulation of mutant fibroin light chains induces an unfolded protein response in the posterior silk gland of the Sericin cocoon Nd-sD strain of silkworm Bombyx mori.2017

    • Author(s)
      Tadashi Takahashi, Masao Miyazaki, Shin-ichiro Kidou, Yoshiki Matsui, Ying An, Taku Ozaki, Koichi Suzuki, Tetsuro Yamashita.
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 86 Pages: 105-112

    • NAID

      130006223753

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The protection of rat retinal ganglion cells from ischemia/reperfusion injury by the inhibitory peptide of mitochondrial μ-calpain.2016

    • Author(s)
      Taku Ozaki, Tetsuro Yamashita, Hiroshi Tomita, Eriko Sugano, Sei-Ichi Ishiguro.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 478(4) Issue: 4 Pages: 1700-5

    • DOI

      10.1016/j.bbrc.2016.09.006

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ミトコンドリアにおける新規カルパイン分子種の同定2018

    • Author(s)
      岩本 健, 石山 絵里, 石田 欣二, 山下 哲郎, 尾﨑 拓
    • Organizer
      日本生化学会 東北支部 第84回例会・シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] ブタ網膜を用いたex vivo緑内障モデルの作製2018

    • Author(s)
      佐藤 翼, 山下 哲郎, 尾﨑 拓
    • Organizer
      日本生化学会 東北支部 第84回例会・シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] 未知ミトコンドリアカルパインの探索2018

    • Author(s)
      菅原 優, 長田 悠佳, 山下 哲郎, 尾﨑 拓
    • Organizer
      日本生化学会 東北支部 第84回例会・シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] ミトコンドリアm-カルパイン阻害ペプチドによる細胞障害メカニズムの解析2018

    • Author(s)
      大河内 裕史, 尾﨑 拓, 宮崎 雅雄, 山下 哲郎
    • Organizer
      日本生化学会 東北支部 第84回例会・シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] ミトコンドリアカルパイン-1の新規基質分子の探索2018

    • Author(s)
      永島 昂, 大内 史子, 山下 哲郎, 尾﨑 拓
    • Organizer
      第91回 日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 細胞膜透過性Tat付加ペプチドによるラット網膜およびHela細胞への影響2018

    • Author(s)
      大河内 裕史, 尾﨑 拓, 宮崎 雅雄, 山下 哲郎
    • Organizer
      第91回 日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] レンチウイルスベクターを用いたアポトーシス阻害ペプチドの発現系の開発2018

    • Author(s)
      及川 建矩、福田 智一、尾﨑 拓、宮崎 雅雄、山下 哲郎
    • Organizer
      第91回 日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Ion Channel Properties of Cells Expressing Two Different Types of Channelrhodopsin Genes.2017

    • Author(s)
      Hiroshi Tomita, Eriko Sugano, Kitako Tabata, Yoshito Watanabe, Taku Ozaki, Makoto Tamai.
    • Organizer
      ARVO 2017 Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mitochondrial Calpains and Apoptotic Cell Death.2017

    • Author(s)
      Takashi Nagashima and Taku Ozaki
    • Organizer
      Joint International Symposium on Science and Technology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cytotoxic mechanisms of mitochondrial m-calpain inhibiting peptide.2017

    • Author(s)
      Hiroshi Okochi, Tetsuro Yamashita, Taku Ozaki
    • Organizer
      2nd International Symposium on Innovations in Plant and Food Sciences
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ミトコンドリアm-カルパイン阻害ペプチドの細胞障害機構2017

    • Author(s)
      大河内 裕史、尾﨑 拓、宮崎 雅雄、山下 哲郎
    • Organizer
      日本農芸化学会東北支部 第152回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Cytotoxic mechanisms of mitochondrial m-calpain inhibiting peptide.2017

    • Author(s)
      大河内 裕史、尾﨑 拓、宮崎 雅雄、山下 哲郎
    • Organizer
      第90回 日本生化学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Analyses of novel substrates of mitochondrial calpain-1.2017

    • Author(s)
      尾﨑 拓、永島 昂、山下 哲郎
    • Organizer
      第90回 日本生化学会大会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2023-03-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi