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Analysis of cytomegalovirus replication in organ-cultured cornea and genotypes of cytomegalovirus coneal endotheliitis

Research Project

Project/Area Number 16K20304
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionGunma University

Principal Investigator

Hosogai Mayumi  群馬大学, 医学部附属病院, 助教 (20760177)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsサイトメガロウイルス / 角膜内皮炎 / 眼感染症 / ぶどう膜炎 / 次世代シーケンス / 器官培養 / PCR / 遺伝子型
Outline of Final Research Achievements

The clinical evidence for cytomegalovirus (CMV) corneal endotheliitis in immunocompetent patients has continued to accumulate, but it remains to be confirmed whether CMV can replicate in corneal endothelial cells. We have obtained the finding that CMV laboratory strains could replicate efficiently in primary cultured corneal endothelial cells. In this study, we examined growth pattern of CMV in corneal epithelium, stroma, and endothelium using organ-cultured human corneas. All CMV laboratory strains could replicate, and expand horizontally in the corneal endothelium and perpendicularly to the corneal stroma. In order to compare the genotype of the CMV laboratory strains, we attempted to sequence for the CMV genotyping using the small amount of DNA extracted from intraocular fluid of the patients. So far, we have amplified the CMV genome and accumulated sequence data. We are continuously analyzing the genotyping of CMV focusing on the indispensable genes.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Journal Article] Corneal endothelial cells activate innate and acquired arm of anti-viral responses after cytomegalovirus infection2017

    • Author(s)
      Miyazaki D, Uotani R, Inoue M, Haruki T, Shimizu Y, Yakura K, Yamagami S, Suzutani T, Hosogai M, Isomura H, Inoue Y
    • Journal Title

      Exp Eye Res

      Volume: 161 Pages: 143-152

    • DOI

      10.1016/j.exer.2017.06.017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic analysis of varicella-zoster virus in the aqueous humor in uveitis with severe hyphema2017

    • Author(s)
      Hosogai Mayumi、Nakatani Yoko、Mimura Kensuke、Kishi Shoji、Akiyama Hideo
    • Journal Title

      BMC Infectious Diseases

      Volume: 17 Issue: 1

    • DOI

      10.1186/s12879-017-2518-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cytomegalovirus retinitis followed by immune recovery uveitis in an elderly patient with rheumatoid arthritis undergoing administration of methotrexate and tofacitinib combination therapy2017

    • Author(s)
      Yanagisawa Kunio、Ogawa Yoshiyuki、Hosogai Mayumi、Todokoro Daisuke、Mitsui Takeki、Yokohama Akihiko、Kishi Shoji、Handa Hiroshi
    • Journal Title

      Journal of Infection and Chemotherapy

      Volume: 23 Issue: 8 Pages: 572-575

    • DOI

      10.1016/j.jiac.2017.03.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] サイトメガロウイルス感染と眼疾患2017

    • Author(s)
      細貝真弓
    • Journal Title

      眼科

      Volume: 59 Pages: 37-46

    • NAID

      40021066831

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] 眼炎症性疾患におけるヘルペスウイルス研究奮闘記2017

    • Author(s)
      細貝真弓
    • Organizer
      第15回新潟女性眼科医の会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] サイトメガロウイルス前部ぶどう膜炎におけるガンシクロビル耐性変異株の検索2017

    • Author(s)
      細貝真弓、中谷陽子、鈴木崇、高瀬博、杉田直、白石敦、秋山英雄
    • Organizer
      第71回日本臨床眼科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サイトメガロウイルス陽性続発緑内障の隅角組織におけるウイルスDNA検出と病理組織学的検討2017

    • Author(s)
      細貝真弓、中谷陽子、濱中輝彦、高瀬博、横尾英明、秋山英雄
    • Organizer
      第64回 北関東医学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サイトメガロウイルスは線維柱帯細胞に感染するか?2017

    • Author(s)
      清水大輔、宮崎大、清水由美子、細貝真弓、磯村寛樹、井上幸次
    • Organizer
      第121回日本眼科学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サイトメガロウイルス陽性続発緑内障の病理学的検討2017

    • Author(s)
      細貝真弓、濱中輝彦、高瀬博、横尾英明、秋山英雄
    • Organizer
      第121回日本眼科学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] サイトメガロウイルス角膜内皮炎の病態解明をめざして2016

    • Author(s)
      細貝真弓
    • Organizer
      2016年度Core Network of Ocular Infection学術講演会
    • Place of Presentation
      ヒルトン大阪(大阪府・大阪市)
    • Year and Date
      2016-11-19
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] サイトメガロウイルスと眼疾患2016

    • Author(s)
      細貝真弓
    • Organizer
      第53回日本眼感染症学会
    • Place of Presentation
      東京国際フォーラム(東京都・千代田区)
    • Year and Date
      2016-07-02
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 次世代シークエンサーによる眼内液由来の微量なDNAをもちいたヒトサイトメガロウイルス全ゲノム解析の試み2016

    • Author(s)
      細貝真弓、秋山英雄
    • Organizer
      第57回日本臨床ウイルス学会
    • Place of Presentation
      ホテル華の湯(福島県・郡山市)
    • Year and Date
      2016-06-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 緑内障手術を要したサイトメガロウイルス陽性続発緑内障の臨床像と病理学的検討2016

    • Author(s)
      細貝真弓、山口由美子、秋山英雄、中谷陽子、磯村寛樹、横尾英明、濱中輝彦、岸章治
    • Organizer
      第30回ヘルペスウイルス研究会
    • Place of Presentation
      セミナーハウス クロス・ウェーブ府中(東京都・府中市)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Activation of cytotoxic T lymphocytes by corneal endothelial cells after infection of human cytomegalovirus2016

    • Author(s)
      Ryu Uotani, Dai Miyazaki, Yoshitsugu Inoue, Mayumi Hosogai, Tatsuo Suzutani
    • Organizer
      The Association for Research in Vision and Ophthalmology Annual Meeting
    • Place of Presentation
      Seattle (USA)
    • Year and Date
      2016-05-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 次世代シーケンサーを用いた眼内液由来ヒトサイトメガロウイルス全ゲノム解析の試み2016

    • Author(s)
      細貝真弓、中谷陽子、高瀬博、杉田直、秋山英雄、岸章治
    • Organizer
      第120回日本眼科学会総会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-04-08
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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